We narrowed to 938 results for: Kin

1. Hot Plasmids - October 2020

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   ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

2. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...specific gene or brain region. When it comes to making the genetic manipulation, there are two main decisions...should be used? Modifying gene expression Generally speaking, one may want to achieve either inhibition or ...

3. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...engineered mice: transgenic mice, knockin and knockout mice, and conditional knockin or knockout mice. While these...

4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

5. Adapting Toehold Switches to Detect Zika Virus

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   ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

6. The Materials Science of Optogenetics Experiments

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   ...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

8. The AAVantages of AAV in CRISPR Screens

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   ...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

9. Magnetic Control of Proteins: More than a Dream

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   ... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

10. Pooled Library Amplifications

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    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...