We narrowed to 938 results for: Kin

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   ...and could be useful to labs wishing to create this kind of animal models. Start using adenoviral vectors...and also Adeno Cas9 and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find...

2. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   ...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...ties directly back to your specific goal. When thinking about step 3, however, you may be surprised at...

4. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

5. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   ... are likely to order several plasmids at once, breaking the statistical independence assumption of the... biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the Faculty at the ...

7. DNA Purification Without a Kit

   Type

   Blog Post

   ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

8. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   ...absorbance strength is low compared to other reagents, making it more difficult to detect. A particularly strong...applications and that you use the same solution for blanking your machine!  Finally, many NanoDrop systems ...

9. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

10. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...