We narrowed to 1,073 results for: TOR;

...reduced by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...ladder runs off the gel. Luckily, this is easily monitored in real time, as the dye front (and many ladders...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...longer time Protein degradation Add protease inhibitors to lysate Insufficient denaturing conditions...

...to sort cells based on the expression of a new receptor that is of interest to you. You found a couple...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...understand if protein:protein interactions could be a factor in your analysis. Example: You are planning an ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

...protocols for plasmid cloning and working with viral vectors, but what we lacked were videos guiding completely...completely new members of the lab or those taking introductory lab courses. The Intro to the Lab Bench series... need a refresher on some best practices for introductory science techniques. It’s also great for teachers...

...improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...

...new primary investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit...

... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...

...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ...

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...'ve helped her at Novartis. The importance of mentorship in developing career skills. Outro Many thanks...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your...