We narrowed to 971 results for: lars;
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TypeBlog Post... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...
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Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature... -
Antibodies 101: Affinity Tags
TypeBlog Post...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...writer When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing... -
Management for Scientists: Giving Feedback
TypeBlog Post...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...attacks. He described one such recent attack as "hilarious". Welcome to Montana! Though sadly I will not ...components, and challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols... -
Intro to Our Summer SciComm Series!
TypeBlog Post... all be defined as laypeople when it comes to molecular biology. Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people...