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  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...
  2. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...
  3. A Guide to Starting Your Own Journal Club

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    Blog Post
    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...
  4. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...
  5. Plasmids 101: Plasmid Incompatibility

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    Blog Post
    ...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...
  6. How to Write a Scientific Review Article

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    Blog Post
    ...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...
  7. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...
  8. Transferable Skills Guide: Conflict Resolution

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    Blog Post
    ...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...
  9. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...
Showing: 761 - 770 of 1074 results