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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 819 results for: cell

Showing: 761 - 770 of 819 results

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...reagent. Be aware that this approach requires excellent attention to detail, since you’ll end up with ...question before running one.  Good on gradients? Excellent! Now, let’s head west, intrepid reader! Protein...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...to see if one works better than another. For nitrocellose membranes, you can even give gelatin a shot....strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

  3. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...expecting a salary increase have had that increase cancelled. Rounding out the remainder, 2% of postdocs who... are continuing with their plans, and 17 have cancelled their plans. 2 of those raising - the University...Illinois and the University of Michigan - initially cancelled their plans, but have since reversed their position... In addition, PIs at some of the institutions cancelling plans are going ahead with raises anyway, including...injunction, as we started to hear of institutions cancelling plans to raise salaries, the only postdocs we...

  4. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

  6. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...transferable skills while you are also learning to be an excellent scientist. Leadership and management Take a lead...supportive editors so your first drafts are always excellent Technical knowledge and creativity Become a “...local networking and trade organizations Another excellent perspective on this topic can be found on the ...

  7. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...give you a chance at an interview:  1) Have an excellent resume ready to be submitted The structure and...successful application. It takes time to prepare an excellent resume…it is worth it. Read here to get help if...if you are having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly...

  8. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...is an excellent resource that has gained a lot of traction with researchers. It is an excellent way to...programs and departments Graduate school was an excellent time to try new things, develop new skills, and...

  9. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...threonine-specific protein kinases. They regulate diverse cellular programs by acting as an integration point for...

  10. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics Background Förster ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses...
Showing: 761 - 770 of 819 results