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We narrowed to 1,073 results for: TOR;

Showing: 781 - 790 of 1073 results
  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...
  2. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...
  3. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...
  4. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... discussed them in meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this... a lot on context. New people in the lab or collaborators will not have the context you do, which means...
  5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...GBPs together to create a complete transcription factor, activating luciferase transcription. Tang et al...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...
  6. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ...aligns with your work, your advisor may want to mentor you during the grant application process. When ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...write your first grant proposal" by Soapbox Science Editor in Nature Blogs "How to write a research project...
  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working...
  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...NG  - SpRY nuclease  C to T Base Editor SpRY A to G Base Editor SpRY   NYN/NRN NYN is less effective...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...
  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...
  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...improved search algorithm which was trained on key factors from past PubMed user searches, such as clicks ...other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers. These...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...
Showing: 781 - 790 of 1073 results