We narrowed to 971 results for: lars;

1. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ... this process, you will first need to do some molecular cloning. Depending on whether you are trying to...send your plasmid to a company. For a few hundred dollars, they will inject the plasmid into fly embryos ...

2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   ...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization during ligand detection...

3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   ...light-based control of signaling pathways. This is particularly exciting because using light to control signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

4. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   ...systems, methyltransferases, methylate DNA at particular sequences to prevent them from being degraded...Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...re happy to provide them, that never works on a large scale. The world is too big now. Mendenhall: So ...on many other labs that may be doing something similar. There may be other variants of this that are better...

6. dTAG - You're it!

   Type

   Blog Post

   ...proteasome machinery to degrade any protein. We are particularly excited about the dTAG system as we can use ...broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

7. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   ...inhibition, with TRIM-away targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21... protein and the target antibody works well. In larger cell populations, you can deliver these components...

8. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   ...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...composition available. If you’re going to need a large quantity of antibody for your experiment, you should...

9. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...edited embryos proceeded to the blastocyst stage at similar rates to untreated controls. Ma et al. performed...germline editing. Although this paper represents a large step forward in the CRISPR field, it’s important...

10. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With six figures....and/or apprehensive. I still get nervous quite regularly, and I've had multiple jobs where all I did was...