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We narrowed to 653 results for: EPO

Showing: 71 - 80 of 653 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    ...use of both when making plasmids. Use repositories Repositories such as Addgene help share materials quickly...After the great Plasmid Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid...in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations for any...clear that community engagement with and use of repositories like Addgene provides large-scale savings. How...and the 2018 numbers for consumable costs, our depositors have helped us save the scientific community ...on Addgene.org Join the community of Addgene depositors here Check out our special collection pages to...find plasmids Search for a plasmid in Addgene’s repository ...

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations...GFP. They then tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells ...tested in retinal electroporation studies, DsRed reporter expression was induced in ~76% of GFP+ cells, ...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...fluorescent reporter, directly couple silencing of your gene target to silencing of the reporter. This is...fusing your gene target sequence to a fluorescent reporter sequence (discussed below). Examples of these ...contains a target sequence against the fluorescent reporter in an inverted repeat orientation. Therefore, ...sequence against your gene(s) of interest and the reporter. This approach greatly enhances experimental throughput... observable phenotypes quantified. Fluorescent reporter-based RNAi constructs have been designed and widely...processes to isolate RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological parameters...identified as undergoing RNAi through the fluorescent reporter and manually extracted. This process is tedious...

  4. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed ...introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered...library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library here A new...  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible CRISPRa system...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...unique skills and strengths (you will likely have reports with different levels of experience), find out ...tasks before I was a manager! Second, my direct report was not engaged because I only left him/her with...you’re delegating a recurring task to a seasoned report, there should be questions! Remember that delegating...itself is counter-productive. I always assume that reports want to succeed at work and providing feedback ...feedback is needed for that. In many cases reports are not aware of issues until you bring them up. I wouldn’...wouldn’t go as far as saying that your reports will be grateful for the feedback, but at least you can start...someone’s hard work and reward a job well done. Your report will (needs to) feel valued. Talk to your manager...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ... 2A element (depending on the placement of the reporter gene). Addgene's collection offers a variety of...multicistronic element(s), or you could replace one of the reporter genes above with your gene or genes of interest...equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released: Addgene's...

  7. New Viral Vectors - March 2024

    Type
    Blog Post
    ... our viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21...vectors that can constitutively express JEDI-2P, deposited by the St-Pierre lab. Also of interest is the ...check out Addgene's Data Hub to view viral vector reports from other users. And don't forgot to use the link...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... people usually report? Addgene and other viral vector manufacturing facilities report the physical titer...cell transduction assays. Wild-type AAV2 has been reported to have a near-perfect physical-to-infectious ... However, for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:...(“full”) (Zeltner et al., 2010), while another reported that only 20% of recombinant AAV2 particles were...vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...AAV aliquots. Our titer values (circled in red) report physical titer, which is measured by qPCR. ...nonsense to publication quality data." Scientific Reports 7 (2017). PubMed PMID: 28546538. PubMed Central...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since...fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found to be a...E., Sivanesan, D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein...Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions...evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu, D. et al...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...research. If you’d ever like to deposit a kit, please contact us at depost@addgene.org. ...of the many useful kits you can order from the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators... an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37. pCri...quantitatively determine allele frequencies across timepoints or populations. This kit consists of a 48 plasmid...fusions. Some applications of PUF protein fusions reported in the literature include RNA detection, RNA cleavage...
Showing: 71 - 80 of 653 results