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We narrowed to 643 results for: ATO

Showing: 141 - 160 of 643 results

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... Figure 6: Diagram of ovary and oviduct anatomy with the equipment used for i-GONAD. A micropipette...  Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with...Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka. “Easi-CRISPR for creating knock-in and...10.1038/nprot.2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline...

  3. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light within a specific range of wavelengths. Excitatory channels allow positively charged ions such as...combine excitation and inhibition by using both excitatory and inhibitory channels activated at different... the opsin.      Figure 2: Diagram of excitatory (ChR2) activation by light showing ions flowing... a single pathway to activate various protein regulators at different points in the pathway. Photoswitches...., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 58112 tdTomato-MAPTau-C-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58113 tdTomato-MAPTau-...TARDBP GFP CMV ALS Zuoshang Xu 28205 wtTDP43tdTOMATOHA TARDBP HA, tdTomato CAG ALS Zuoshang Xu 28206 TDP43 ...ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich ataxia Richard Possemato 102975 pLKO.1P...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...tdTomato-MAPTau-N-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58259 pBabe-Neuroserpin SERPINI1 Dementia Joan....1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448...James Trimmer 182922 hSOD1-FLAG SOD1 CMV ALS Philip Eaton 183984 huntingtin:TOC031-A07:C246751 HTT Flag CMV...

  5. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot...responses to cancer. The screen recovered known regulators, such as Ptpn2, Pdcd1, and Zc3h12a, demonstrating...Moreover, the screen identified putative novel regulators of CD8+ T cell responses in tumors, demonstrating...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...makes time for you, as needed, this is a strong indicator that this person cares about your science and ...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...customized to control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... frequently been used for screening candidate regulatory elements such as enhancers and promoters for ... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....., … Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(...

  8. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...

  10. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive measures...Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is ...a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal precision...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other...

  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...activation of keratinocytes, which release inflammatory mediators that trigger the activation of dendritic...Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6...IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both respond to and release...

  13. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...CRISPR for that, too! CRISPR activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The...increase expression. There are several flavors of activators which can be used with this approach - read more...them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms Want to transiently...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    ...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular... disorders, including some forms of lupus and rheumatoid arthritis. These types of immune responses are...

  15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...available to counteract the toxin. If, however, an investigator were to successfully clone an insert into the...insertion will completely replace ccdB with the investigator’s insert of interest. Hence correct clones are... behind the “swapping out” of ccdB with the investigator’s gene of interest.   CcdB resistant E. ...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031...

  16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can...expression robustly activated the reporter gene TdTomato, whose expression was absent without electroporated...T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a high activation...frequency of 56-93%. In the converse test, 98% of TdTomato expressing cells were GFP+, indicating a robust...

  17. Plan Your Experiment

    Type
    Collection
    ...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...promoter elements of target gene Many different activators exist, including the multi-plasmid SAM system. Select...activate or repress a target gene using dCas9-activators or dCas9-repressors, gRNAs should be targeted...

  18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...bone marrow (BM) cells, primary human hepatocytes, and hematopoietic stem cells (HSCs) using Nanoblades....deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...from GFP transgenic mouse  GFP  ~60-65% Human hepatocytes  Myd88  ~50% Human HSCs  Myd88  ~50% Table...

  19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential... 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because, at high concentrations...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ..., 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator, which can control output...cable is threaded through—and fixed to—an optical insulator called a ferrule (below). The fiber optic cable...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...
Showing: 141 - 160 of 643 results