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We narrowed to 728 results for: STO

Showing: 211 - 240 of 728 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent...

  3. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ... done at my first six ASM meetings. I told this story over a great dinner at Pittypat’s Porch with old... career scientists, students, and those from historically underrepresented groups. Through such mentoring...Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa ...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...to inject an Agrobacterium culture through the stomata into the abaxial site (lower surface) of the leaf...MoClo system you can easily and quickly create customized vectors. For more information on MoClo for plants...is a PhD candidate at Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology...

  5. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... payoff for each aim. Learn to communicate your story with only the most salient details. Make the most...your research problem. Creating a coherent unified story and model can help make your ideas easier to understand...Practice presenting your chalk talk Perfect your story and delivery. Design simple figures and diagrams...track. Furthermore, practice getting back to your story. Committee questions can derail your talk. You need...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag...Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P....

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...infectious titer. The infectious titer of a viral vector stock can vary based on transduction target and can also...particles were full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-...100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center for Nanoscale Systems, Harvard ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially... Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein FRET: the good...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...key to fitting large genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...truncated protein. This can be avoided by excluding a stop codon from donor A in any of the reading frames ...the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick ...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic... blot, at <10% of the wild-type level. Muscle histology is improved, with vastly reduced inflammation ...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant...OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed...

  13. A Control for All Seasons

    Type
    Blog Post
    ...Stephen M., et al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. ...Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications...and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission to the British Journal...

  14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...relationship for the mentee, where (s)he feels truly understood and supported, and can more fully identify with...University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk ...

  15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Cell lines and reagents Examine your cell line stock for mycoplasma contamination before use. Make sure...using expired products or reagents that have been stored for a longer time. Use fresh media as much as possible...for prevention. Prepare a small quantity of media stock that would be used for the experiment, preventing...line to another. Multiple cell lines should not be stored in a single incubator since it increases the risk...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...undergraduate years. We each have a unique “origin story,” but taking the first step into the world of academic... to find out what a “postdoc” is, and that “PI” stood for principal investigator. In basic science (as...after earning a doctorate Considered a "stepping stone" position to become a professor, or other scientific...the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science Careers discusses ...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...into inserting a fluorophore-tagged amino acid at a stop codon (often amber i.e. UAG) engineered into a desired...suppression competes with natural chain termination at stop codons, expression levels of these tagged proteins...Research department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent...British anti-Lewisite (dimercaprol): an amazing history." Annals of emergency medicine 41.3 (2003): 378...

  18. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...in grad school was that scientists have amazing stories to share. I spend a lot of time telling scientists...for genome editing? Know a professor with a great story to tell? Let us know by emailing us at [email protected] on Twitter and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.   ...

  19. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...cellulosic biomass to useful products under Professor Christopher E. French. Dr. Duedu was recently awarded plasmids...plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at the University of Edinburgh...opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many thanks...

  20. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... online access on scientific impact Partly, the story has to do with online accessibility. It is now about... recently reported that the most cited work in history is a 1951 description of an assay to determine ...of scientific publications. Ventures like Impact Story seek to find ways to capture scholarly work that...

  21. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Research) – and an Addgene depositing scientist – stop by our booth to say hi! Zhang was awarded one of... to help their labs save time and resources by storing and distributing their plasmids with the scientific...our booth, I will have more updates coming soon. Stop by booth #3431 if you’re at #SfN14 and meet our ...

  22. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite...reading this article - you should join Dr. Bourne in Boston on October 2nd when he will be the keynote speaker...

  23. Let There Be LITE Plasmids

    Type
    Blog Post
    ... entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like...expression via epigenetics with the addition of histone modifiers. “We want to allow people to prove the...476 (22 August 2013).   , with the addition of histone modifiers, ...

  24. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... By Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are ...clarify the situation; C = confirm that you have understood the speaker accurately. Thank employees who volunteer...by feedback. If you don't do this, employees will stop providing feedback pretty quickly.   This is the...

  25. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for protein...bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may...

  26. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... can edit the documents and track the revision history. Now you’ll be able to make updates to protocols...consider moving your paper spreadsheets for reagent storage locations, such as the list used to organize your...samples. Once you've taken some time to get organized, stop back and let us know what worked for you. Share ...

  27. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...was immediately interested in applying. This had stood out to me because it gave me a full description ...clear that Addgene wanted to ensure applicants understood what the expectations were for the intended position...make sure people feel connected.    As I became accustomed to the routine, I realized that the “9-5” was...

  28. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...postdoc-driven Future of Research Conference at Boston University last week.  This fantastic city-wide... supporters from all of the research centers in Boston.  I was relieved because it finally feels to me...program.  His strongest comments were about the astounding lack of reproducibility being recognized in published...

  29. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...should be able to tell your story for you and they should also be easily understood and consistent in design...Writing and Publishing Institute. Writing is the cornerstone of any scientist’s career. We use writing to ...
Showing: 211 - 240 of 728 results