We narrowed to 498 results for: plasmid dna

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...transducing a only a single gRNA per cell. The viral DNA containing the gRNA then integrates into the genome...identified by next-generation sequencing of the genomic DNA. However, some cell types are poorly transduced by...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...

...connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of DNA parts ...Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a ...background MoClo is a clever way to assemble several DNA parts in a specific orientation using Type IIS restriction...between CRISPR components expressed from different plasmid backbones. They noted that backbone differences... as the order, or distance in the placement of plasmid elements can impact expression levels and consequently...system was used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare...plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters or fluorophores are ...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ...sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein...segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just...your protein knockdown experiments! The TRIM-Away plasmids can be found here. Clift et al. Cell. 2017. PubMed...Endosomal pH Sensors podcast segment The first Addgene plasmid deposit from Professor Stefan Hell, who won the...

... “multiple DNA fragments in an ordered fashion in a single reaction.” Using these plasmids, which are ...Guide to TALEN technology and using TALEN plasmids TALENs...Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in...Imagine being able to manipulate a specific region of DNA in the genome of your favorite species almost as ...biological disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of ...enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists have identified...expect that assembling relatively small, repeat-laden DNA regions into a single construct would be technically...

...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA....precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments of DNA at specific...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases...guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless", as...homology sequences are integrated along with the donor DNA. The two main classes of CASTs are type I and type...

...-target DNA editing activity. In fact, it also reduced the (very low) levels of off-target DNA editing...Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature, 551(7681), 464–471. https...editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 533(7603), 420–424...was invented in David Liu’s lab by combining Cas9 DNA-targeting technology with a fused cytidine deaminase...editors did not create double-stranded breaks in the DNA backbone, making them significantly less prone to... using directed evolution to get them to work on DNA instead. However, they retained a small amount of...Analysis and minimization of cellular RNA editing by DNA adenine base editors. Science Advances, 5(5), eaax5717...

...letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX...insert in your plasmid name. This is often a 3-6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene...information about plasmid features and tips you can use in the lab, check out more posts in our Plasmids 101 series...There are no universal rules for naming plasmids but here are some good guidelines to follow in order...people can quickly and easily identify what your plasmid contains and other important information. Tip:...Backbone name Include the empty backbone name in your plasmid name. This simple piece of information can often...important details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial...

...can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...that describe methods to make single base edits in DNA. The first, contributed by guest blogger Kristian...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service A fast blue-light...recognize, cut, and alter specific target sequences in DNA resulting in a variety of outcomes including excision...

...- that is, since you’re looking at the amount of DNA in your sample relative to your normal control - ...dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with a fluorescent...The fluorescent signal can be affected by AT-rich DNA and the length of the amplicon and it has a higher...In both applications, results can be affected by DNA quality and primer design, particularly for amplicons...statistics), and the readout gives an absolute value of DNA concentration. By comparing the number of positive... number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length. Am J Trop Med... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...

...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...liquid DNA with all of the plasmids in the library in a single tube. The number of unique plasmids in a ...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

...interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, such ...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...institutions have proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable cell...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...antibody can be converted into recombinant form through DNA sequencing of the line and subsequent cloning of ...

...vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your colleagues...Virus-associated DNA Each viral vector service request will also include virus-associated DNA, which is a ...a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...widely cited in publications. All pricing includes DNA amplification, viral vector production, purification...inclusion is what gives you permission to use the plasmid....

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...

....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells...

...CRISPR plasmids that create double strand breaks in DNA....genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and a specific...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...ddPCR) Historically, a quantitative DNA hybridization method (DNA dot blotting) had been used to titrate... as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron...concentration of viral genomes (by qPCR or other DNA quantification methods - see below), since each viral...concentration. We regularly make and validate fresh plasmid standards. We validate our absolute quantification... digital PCR system for absolute quantitation of DNA copy number." Analytical chemistry 83.22 (2011): ...

...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that 41/42 embryos...editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, it will ... homozygous mutations. Moreover, introducing new DNA seems impossible with current tools, thus quieting...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

... its reported cleavage site on the complementary DNA strand. And this is why we love working with researchers... have in order to scan along the double-stranded DNA, recognize a PAM sequence, allow the complementary...base pairing between the guide RNA and the target DNA strand, and then activate two different endonuclease...This structure captures the displaced non-target DNA strand and shows the HNH nuclease domain swung around...juxtaposition to its cleavage site on the target DNA strand. In addition, this model also displays the...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ... technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making models more...

... Addgene/Seeding Labs plasmid grant and plans to use the grant to order plasmids she can use as positive...similar grants back in June, Gladys will be using plasmids in creative ways that we wouldn’t necessarily ...commonly used in Ghana, there is little screening for DNA markers like the presence of high risk (HR) Human...HR HPV types, predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys...cytological tests currently in use. Gladys will use plasmids containing a variety of HPV genes as positive ...incredibly happy to hear that Gladys will be using plasmids in a way that can potentially have a direct impact...awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of applicants at the start...

...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety of applications...transposon called Tol2 to randomly integrate foreign DNA into the genome. Non-random integration into specific...facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...collection Read our molecular biology reference Find plasmids for your research ...

...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....incredibly important role in solving the structure of DNA. 2. The Immortal Rap of Henrietta Lacks Students...Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ... repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should ...module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2... TALE plasmids. Genome...

...Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...

...Isotype switching is caused by a specialized type of DNA recombination. Heavy chain constant genes are clustered... expressing a different isotype, the intervening DNA sequence is deleted, which places the variable region...    Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. B cells...in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain ...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

...the chimera are encoded by DNA. Since researchers can generate almost any DNA sequence in the way that ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...protein over-expression artifacts The introduction of DNA that encodes a fusion protein adds protein to the...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...

...genome is lethal. If this DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which...sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, ...genes in E. coli using plasmid and phagemid delivery systems. Phagemids are plasmids containing a phage origin...sequence results in cell death. Plasmid targeting may result in plasmid loss and antibiotic resensitization... three essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target...this resistance gene being encoded by a high-copy plasmid. In the case of the genomically encoded quinolone...resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for ...

...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They might...of molecular biology, many scientists do a lot of DNA work but never get to actually manipulate the organism...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal) The Hawaiian...efficiently transform these cells which don’t like foreign DNA at all (maybe because they have only 1,700 genes ...

...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break....Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ..., creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been ...fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage ...