We narrowed to 990 results for: Abo

... the best way I’ve come up is this: It takes me about the same amount of time to read a 100-page genre...it instead as valuable feedback.  Listen. Think about the questions you’ve received, read the comments...aware of cognitive load changed the way I thought about engaging with learners. If something I was communicating... instead of blaming the audience, I would think about how I was presenting the information. Maybe I needed...observe and adjust on the fly.  Once you can think about everything you’re asking your learners to do, it... engagement with your target audience. Thinking about cognitive load will help you understand why you’...

... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

...differently based on their audience. How you talk about your experiments to your PI, your colleagues, or...have cross-team projects within your own lab, collaborations with other scientists within and outside your...not happen in the lab, they have parallels to collaborative lab work. Corralling context and junking jargon...difficult to know what kind of plate they were talking about during the meetings. And “plate” certainly wasn’... a lot on context. New people in the lab or collaborators will not have the context you do, which means... Additional resources on the Addgene blog Read about other transferable skills Find all of our science...

...20th anniversary was about all the amazing years behind us, as much as it as about the amazing years in...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning...Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also... As the pandemic slowed, Addgene began to think about how we could expand our support for the research...part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative....

...silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in my head...doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week!  Nerves...attention? fear of messing up?–and use the three tips above to make a strategy that can help. Even something...speaking as a transferable skill The worst thing about doing public speaking as a scientist is that you...perfectly, you truly are a rock star.  The best thing about public speaking as a scientist is that you will ...increases visibility of your work, opens up collaboration and growth opportunities, and gives your entire...

...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research...research.  It helps to spend some time thinking about what your goals are for the coaching relationship...ask for references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will have...with someone you connect with. Talk to the coach about their “style” and make sure this is a style that...coaching group is Still Point Coaching. One nice thing about a group is that sometimes this offers the option...

... work into finding robots that’ll take a little labor off our hands. Good news! There is probably a machine...96 transformations in one run compared to doing about 50 transformations in the same amount of time by...machinery we have been able to transform 96 samples in about 4 hours with a greater than 95% success rate! Automating...plates in just under 3 hours or one 96 well plate in about an hour. Automating vial fiIling The majority of...35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand. How can you start...on each tube by hand. We’ll be sure to tell you about more lab updates as we develop them. In the meantime...

...Sainsbury Laboratory. She is passionate about science communications and loves talking about science, ...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...break into science communication? Everybody goes about this in their own way. The career paths into science...Society gives training in media skills, writing about your research and residential communication. If ...

...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that these...finish the library preparation process only takes about one day, even for six plates (576 individual plasmids... we perform a 2x251 run on our MiSeq that takes about two days to complete. After the run completes, we..." In some cases, there will be more information about our sequencing results in the Depositor Comments...to note the appropriate barcode for each sample. About 24 hours later we unload the FASTQ data from our...

...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...output of the laser must be controlled. As discussed above, each opsin variant has slightly different activation...

... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Plasmid cloning is an essential...with successful recombination reported in all laboratory E. coli strains tested so far. The use of this...low cost and can be immediately adopted by any laboratory. All procedures are performed using the same... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology. He is currently investigating...García-Nafría is an investigator scientist at the MRC Laboratory of Molecular Biology. He is interested in neuronal...

...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression..., if not better, than antibodies for all of the above listed molecular techniques, but they can also be...protein purification methods. Read on to learn more about nanobodies and how their structure and function ...between a fluorescent tag and the target antigen to about 2 nm, which can lead to higher resolution for super-resolution...nanobodies. If you’re interested, you can learn more about how nanobodies are identified in this review. If...

...early employees spent this remaining time by elaborately pranking each other, dressing in costume, and...frequently ask the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all day long, whistling...megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring...company.        Additional Resources Read other posts about life at Addgene!Read more of our careers posts! ...

...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...development strategy builds on the steps listed above and formalizes your development. You may consider...

...Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about the viral...resources available from Addgene. There’s a lot to talk about! And by the way, there will be viral vector jokes...injection site, and incubation time. Learn more about the Data Hub in this blog post. Alongside our efforts...SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional resources on...

...disadvantages of this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career ...general experimental guidance, and resolve complaints about products. As you might expect, different companies...order to enhance the OD signals and then complain about high background. In many instances, variations ...protocol. And customers may tell all sorts of details about the application, just not the ones that matter. ...communication, and is particularly excited to hear about novel developments in the fields of gene and cellular...Academic v. Non-Academic Career Decisions Learn about Science Career Options Explore Tips for Technical...

...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...fraction (<1%) of authors have asked for advice about the scope of the policy. End of story? Not quite...the data policy was raised by researchers worried about the effort to make the underlying data easily available...making data available, but there remains concern about the extra work this may entail.  So if access to...be archived? Simplifying data access Some of the above mentioned issues could be mitigated by on-line services...him on Twitter @jwoodgett.     More Discussions About Data Sharing: PLOS Data Sharing Policy Discussion...Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ...

...Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite repression in the ...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and...and operators (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of...this list is a great place to start, the tables above do not delve into the tissue or development-specific...

...screenshot above). Don’t see your favorite organism listed? Help us serve you! Let us know about other organisms...useful plasmid that’s not in the collection? Tell us about it with our Suggest a Plasmid Form! Finally, if... if you would like to get the word out about a particular microorganism, microbial technique, or microbial...microbial technology, sign up to write about it on the Addgene blog. ...

... Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies...Köhler who developed hybridoma technology. But what about single chain fragment variables (scFvs)? scFvs weren...from inclusion bodies, it’s time-consuming and laborious.  To avoid this, one or more of these approaches...antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...Hybridoma Bank Learn more about the SunTag system which uses an scFv to amplify the fluorescence intensity ...

...occurring regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control...Spinach-based riboswitches can be used as stable metabolite sensors for imaging in live cells. By placing...placing Spinach downstream of a riboswitch, metabolite binding to the riboswitch instead activates Spinach ...Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be a better choice for imaging...modification that reduces nonspecific binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach...Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate other fluorescent...

...Baby KJ” was diagnosed with a rare and dangerous metabolic disease shortly after his birth. Within a seven-month...successful base editing intervention was created. Only about half of babies with KJ’s disorder survive long enough...effort was only possible because of a massive collaboration between research hospitals, universities, private...an astounding feat of public investment and collaborative scientific effort. As we like to say at Addgene...activity of adenine base editors The first ABE was about 50% efficient in human cells on average, but its...in the lab. We’re so happy to be a part of a collaborative community focused on CRISPR research, and we...

...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...little prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...

...Addgene Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our Outreach...Allied Sciences in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they plan to...He hopes to teach his molecular biology students about the functions of these powerful tools and to use...China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International Shipping...

... on CRISPR Mouse Models Learn about Homology Directed Repair Learn about Cas9 Ribonucleoprotein (RNP) ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively...process of conventional gene targeting is lowered to about 2-3 months. But certain models remain more difficult... a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months. With CRISPR (B), you...directly into a zygote and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded...

...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ...macromolecules to serve cellular functions. Along with collaborators, the Denes Hnisz Lab previously discovered that...models By Brian O'Neill The Nadav Ahituv Lab and collaborators have found a way to make adipocytes fight cancer...of fat tissue that is able to waste energy by metabolizing glucose to dissipate heat. Of the genes they..., its accessibility outside of well-equipped laboratories remains limited. Members of the Stanley Qi Lab...production, allowing students to explore cell metabolism. Plasmid components of CRISPRkit are now available...

...USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories to start working...where individuals can exchange ideas, network, collaborate, and innovate internationally. Why an International...needs, and came from the universities mentioned above. The initial IMP cohort of 78 mentees included: ...    Other Resources Visit the IMP website Learn about the Mentor Circle Program at Brigham and Women's...Networking for Scientists Series Read our Blog Post about Choosing a Mentor      ...

...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...particularly interesting. Although humans have known about bioluminescent organisms and their characteristic...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...for you, so if you’re interested in learning more about fluorescent proteins, I invite you to download our...

...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

...Addgene blog. In fact, we have so many blog posts about careers that we’ve compiled our top career blog ...wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive Director...school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot ...Usually when I see inspiring artists who are thinking about getting into the arts (or any career to be honest...follow your passion. Do what you love. Don't worry about the rest.”- Jason Snair, Product Designer On the...

...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, ...for rerouting waste from the landfill, but what about all the manufacturing steps that happen before you...ACT label, which is an eco-nutrition label for laboratory products that emphasizes Accountability, Consistency...away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the...

...attracting so much attention. When I spoke with Daniel about the interplay of these two ideas he said “science... scientists sometimes forget how to communicate about their work with non-academic people.” Daniel went...perception, which is ironically what science is about. For me it is essential to stay in touch with the...unique samples, meaning that we know very little about these specimens - especially those which are now...Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on...

...Addgene blog Learn about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...Beauty DNA transposon Learn about the piggyBac DNA transposon ...

...a summary snapshot without telling you anything about outliers or data spread within those data sets. ...the rules” and which we have certain information about. The assumptions and requirements that need to be...Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way to go, which...only performing the comparisons you really care about. Family-wise error is the probability of a false...rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...not meet the assumptions for the tests described above (normal distribution, equal variance, and independent...

...new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is composed... lentiviral expression (MuLE) system from the laboratory of Ian Frew simplifies the process of creating... seem preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative, chaining-based cloning... to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out five rounds...improved light-switchable bacterial TCSs Jeffrey Tabor's lab has developed new synthetic biology tools by...for controlling gene expression. For more details about these light-switchable TCSs, read our blog post:...

... of Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line...all the information in the resource.  This is a labor-intensive project; scripts can be used in some cases...preferences to help you identify proximal lines you care about. If you have cell lines without a certified origin... Or finding errors in the available information about a cell line you’ve used? Submit your manuscript ...distributor. A success all around! If you’re excited about this resource and want to learn more, check out ...

... for bag check was definitely worth it! Hearing about his passion for global health and the various initiatives...with fluorescent microbes. Check out her animation about the Hawaiian bobtail squid!  Continuing the art...talked to some scientists who stopped by the booth about amazing work already represented in the Addgene ...opportunity for Addgene to let microbiologists know about our mission of open science and resource sharing...community, contact [email protected] to find out more about our free deposit and distribution service! Did you...

...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

...living as a scientist in Beijing, talking to people about taking beautiful photographs of their studies, reporting...to friends or dates or colleagues we tell tales about parts of our lives that tend to have a story structure...informational interview question: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee:...situation down by opening a dialogue specifically about the project and contributions, rather than getting...defensive or getting into ‘he said; she said’. We spoke about it for a while and he eventually calmed down, apologised...