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We narrowed to 753 results for: ELP

Showing: 281 - 320 of 753 results

  2. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...horizon for microbiology research and we’d love to help more microbiologists share these valuable resources...our Suggest a Plasmid Form. We look forward to helping microbiologists from all over the world easily ...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...tested, and published by academic scientists. To help you find the vector that fits your experiments, ...message in the comments below and an Addgenie will help you find what you need.   More Plasmid Resources...

  4. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...Round Table. In any field, good communication skills help when it comes to finding a job. Many people in science...SciFund is really motivated more by the desire to help close the widening gap between science and society...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... to have high expression, so adding this G could help with expression, though it is possible for the plasmid...specific plasmids Links to different software to help you identify your gRNA target sequences Additionally...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...translocate or fold in certain conditions? It can help to visualize an abstract protein during your sample...change depending on the conditions and the assay can help you avoid a great deal of frustration when designing...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...original employees at Dimension Therapeutics, she helped build and grow the gene therapy division of the...ultra-rare diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality...

  9. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...subject did you use? For animal subjects it is helpful to report the genetic background and catalog number...number or RRID if it exists. This is helpful because viewers will know exactly which strain you used so...

  10. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...project may not advance beyond its initial stages. Help you build a network outside of the academic world...which skills you should focus on improving and will help reveal potential opportunities beyond academia. ...

  11. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...adding a second stain, safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind...and negative classification system is incredibly helpful in visualizing differentiations between bacteria...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...transduction patterns of different AAV serotypes. To help make this easier for scientists, we now offer trial...catalog item is available for order please email help@addgene.org with the vector name and serotype you...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help to reduce the likelihood of ITR deletions occurring...sequencing results. Next-generation sequencing is also helpful in quantifying ITR mutants since analysis of individual...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    ...other commitment. An announcement of purpose can help everyone to ‘land’ in the meeting and get focused...get stuck diving into details, ask a colleague to help keep you accountable for moving along. Remember,...

  19. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment. The Addgene...catalog item is available for order please email help@addgene.org with the vector name and serotype you...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...chemically reactive side chains, all of which can help determine structure and function relationships or...posting more posts on the various protocols and helping to spread the word as new protocols come out! ...

  21. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...largely does not integrate, and the addition of a helper virus or plasmid allows it to replicate episomally...research, you might have an idea which ones could be helpful to you. Interested in learning more about a specific...

  22. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those sites...visualization software (we used Snapgene here) can help you assess whether your restriction site will be...

  23. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...field will know about grants and projects that might help your work Tenure can depend on your national recognition... and be ready to talk about them. People love to help especially when solving problems.  During the conference...

  25. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...these measurements and hope this information can help guide your understanding of reported data. Photobleaching...substantial changes in excitation power and will help to keep the photostability of the probes in check...

  27. RNA Extraction Without A Kit

    Type
    Blog Post
    ...biotechnology companies have marketed several products that help to further streamline this process and/or stabilize...choose from) on your resuspended RNA sample will help ensure DNA contamination is not an issue.  References...

  28. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...we wish to conduct research that fails less and helps more people, we need to take sex into account. Gendered...Physiology over the past decade. For more practical help, there are numerous methodological articles to consider—we...

  29. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...can use primer design software such as Primer3 to help you design your custom primers. Remember, when designing...forward and reverse primers for each sample with the help of DNA-editing software which integrates our database...

  31. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...family Parvoviridae and requires co-infection of helper viruses like adenovirus for productive infection...and the selection of specific promoters may also help AAV vectors evade host immunity. While basic research...

  33. A Look at Addgene's QC Process

    Type
    Blog Post
    ...it be fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process...the raw NGS reads to a reference sequence can also help QC scientists determine if enough reads were acquired...

  34. Design Tips for Prime Editing

    Type
    Blog Post
    ...details. You can find many software programs to help you design pegRNAs. Here on the Addgene blog, we...a guest blog by the developer of PRIDICT, which helps users select pegRNA designs that are most likely...

  36. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...the practical application of the plasmid and will help a scientist outside your field understand if this...up-to-date version! We hope the deposit spreadsheet will help expedite your data entry and make it easier than...

  38. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...America. Diagnostics will play an important role in helping to monitor and slow this spread until vaccine programs...toehold switches and amplification primers. This helped make sure that we had a number of successful sensors...

  39. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...Exploring the efficiency of these two processes will help us better understand where Cpf1 fits into the genome...

  40. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...learn all about ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet Digital... This is an extremely useful PCR method that can help save time and effort in the laboratory.  There are...
Showing: 281 - 320 of 753 results