We narrowed to 355 results for: Cre expression vector

...initiated. Promoters are a vital component of expression vectors because they control the binding of the RNA...to drive constitutive or inducible expression. Promoter Expression Description T7 Constitutive Promoter...constitutive or inducible expression. Many other promoters can drive expression in specific cells or under...where in the organism your gene of interest is expressed. Promoters are about 100–1,000 base pairs long...under different conditions. Promoter Expression Species Description Ac5 Constitutive Insect Strong promoter...Constitutive Mammalian U6 nuclear promoter for small RNA expression (species-specific versions) CaMV35S Constitutive...Cauliflower Mosaic Virus Ubi Constitutive Plant High-expression promoter from maize ubiquitin gene GDS Constitutive...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood ...lentivirus or use a Cas9-expressing stable cell line. After applying your screening conditions to your transduced...also been used for genome-wide screens to activate or repress gene expression in mice and human cells. The...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...

...entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors deposited...available TOPO-ready, and it is not feasible to create a TOPO vector yourself. Additionally, the efficiency can...activity creates “chewed-back” overhangs of 10–12 base pairs on the 5' end of both the vector and insert...them into plasmids for replication and protein expression. There are many methods that allow you to move...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...shuttled into any compatible Gateway destination vector, which contains attR sites, via LR clonase enzymes...a simple and efficient cloning method, Gateway vectors and recombination enzymes can be quite expensive...

...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...given library have the same backbone, but they express or target different genes. Some libraries cover...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the... Science Guides Addgene has created the following guides to help you start learning...

...parsing the effects of expressing multiple genes at once. A well-designed screen can help you begin to ...information on genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our...given library have the same backbone, but they express or target different genes. Some libraries cover...Negative Screen Negative screens are a little trickier than positive screens. In a negative screen, the goal...plasmid. Library screens can be divided into two types: positive screens and negative screens (Figure 2). ...Types of Screens Resources Pooled libraries represent a powerful tool for forward genetic screening, or identifying...for lentiviral of DNA-based screens. NGS = next-generation sequencing. Created with BioRender.com. Types...