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We narrowed to 915 results for: Tro;

Showing: 351 - 400 of 915 results

  1. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... in a pooled cell population, as mutations with strong phenotypic effects can mask weaker ones. Thus, ... the need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas...them had not yet been functionally validated. In vitro assay allows first-time functional annotation of...collection to screen individual perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and...effect on cell viability compared to a wild-type control. Approximately ~22% of the mutations, however, ...

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids, Blugene...unilateralis is a fungus that infects ants and controls their behavior. These ants become “zombies” and...their jaws into a leaf, and dies as its muscles atrophy. After the ant’s death, the fungal fruiting bodies...display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing it out of hiding...blood out. Once the blood is depleted, the tongue atrophies and falls off. The louse remains becoming the ...

  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist at ...Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption of the smn1 gene...the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind the disease are...restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal Mesothelioma...of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric Neurology...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... The results are then visualized using gel electrophoresis. Surveyor™ and T7E1 have been widely adopted...research setting, but these events could have disastrous health effects in the clinic. NGS-based methods...including Digenome-seq (Kim et al. 2015) for in vitro analysis, IDLV for in vivo detection (Gabriel et...potentially toxic) Digenome Seq 0.1% Cell-free (in vitro) Works across all cell types Must be verified with... background BLESS Not reported Cell-based (in vitro) Can be used on tissue from whole animal models,...most investigators use single cell clones for in vitro CRISPR experiments. The likelihood that a single...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...of input material, cells or tissue, to produce a strong enough signal over background noise. This is not...access to transcriptional regulatory elements is controlled during development using the Drosophila wing ...the target protein. This targeted digestion is controlled by the addition of calcium, which MNase requires...only for a short period of time, thus precisely controlling the amount of cutting and mitigating noise generated...protocol, the most significant being the usage of a strong detergent to permeabilize cells rather than to ...

  6. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    .... Layers of control have also been added to the CRISPR technology to allow time-controlled edits to occur...target can be identified if it isn’t known. If a strong candidate emerges, downstream optimization is performed...generation of knockout cell lines which has made this control step extremely accessible. For example, let’s say...  Susanna Stroik...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...traps can be used as high-throughput screens to introduce reporters throughout an organism’s genome. These...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene...Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids 101 e-book Additional... Susanna Stroik...

  8. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...ways budding young scholars across Europe get introduced to the cultures and opportunities available to... societies to promote their upcoming events and intro sessions, and give out useful items and information...fairs are also where many future scientists are introduced to the biology and chemistry societies that will... to attend. We thought we’d take some time to introduce you to the idea of the Freshers Fair and some ...Scientific societies like those mentioned above provide strong foundations upon which young scientists can build...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...recombination of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular recombination. ...One of the most frequent problems that I help troubleshoot involves viral vectors and plasmids with repeating...testing your prep can save you the headache of troubleshooting failed experiments. Pick the right strain While...backbone.   Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions will help with the next...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...are similar to transfections, processes which introduce foreign nucleic acid into cells by any means, ...safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions can offer...selected to perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired...integrate into the host genome (lentivirus and retrovirus) while others are non-integrating (adenovirus... Susanna Stroik...

  11. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...fluorescent protein reporter under the control of promoters that control cell cycle gene expression.  Additional...genotype the patient and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach...-up approach will allow me to create a fully controllable and reproducible set up,” Bernach explains. ...containing unstable fluorescent proteins under the control of promoters responsive to water or nutrient levels...

  12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...). Before thawing, all the samples were at the control pH of 7.4 as indicated by color (data not shown...thaw. We compared vented and non-vented tubes to controls that were not exposed to dry ice. Does venting...between 0 and 96 hours. We compared these samples to controls that were only stored at -80°C. We then thawed...period of at least 96 hours had the same pH as control tubes that were never stored on dry ice/exposed...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...same correlation, but it wouldn’t be causal. To control for a number of differences between those papers...methodologies, or qualities of research our adjustment controls for them.   So, for example, in 2010, 10 percent...are lots of other factors that could still be controlled for. As an example, plasmid-related areas of ...see such a big difference in citations after controlling for journal. And so, while our quantitative analysis...research tools, computing performance, and randomized control trials in innovation. Visit his website at: http...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ... in the general research community. Phillips Petroleum Company first commercially developed K. phaffii...operates as an obligate aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its...peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model...and NHEJ is an error-prone pathway frequently introducing frameshift knockout mutations. Thus, if genetic... Susanna Stroik...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...streamline identification. While the Zhang lab controlled their multiplicity of infection so that a single...disease depends on both genetic and expression control systems to mimic illness on a molecular level. ...understand a pathway, or just looking for the best astrocyte differentiation protocol! Ready to try it out...either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual plasmids...  Susanna Stroik...

  16. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...skills in strong relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...software product management was either. Coming from a strong background of undergraduate research straight to...energized from interacting with others, and have strong communication skills to tailor your message to ... the details, but the developers have it under control. 9:30 AM--Back-to-back meetings. Meet with my boss...

  17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...go of the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA...that expresses gene III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed ...the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection results...editing speed. Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor... activity. To mitigate this drawback, the lab introduced an additional mutation (V106W) into TadA-8e and...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... use amplicons in ITR as we have found this to strongly bias titer. We are also adopting ddPCR, which ...the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and would like to get it working in...transfection efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections? A: We...difference in AAV transduction efficiency between retro-orbital versus tail vein injections.   Q10: Have...labs have tried to transfer PHP.eB's enhanced CNS tropism to other serotypes, but reproducible success remains...

  19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although this method shortens construct...requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may have off-target... of gRNA cleavage.   Xie et. al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms ...Bacteria and viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...type of genome editing tool with which one can introduce changes into the genomes of mammalian or other... create a pair of designer proteins that will introduce a double-strand break in the genome anywhere you...double-strand breaks, there is a possibility of introducing a mutation somewhere else in the genome. It remains...

  21. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options and cloning...transcript You can also multiplex gRNAs via a polycistronic transcript. Rather than being transcribed from...option described below, plasmids for making polycistronic gRNAs are also available from the Yang lab for...cannot be released, adding temporal and/or spatial control to the system. pSQT1313 from the Joung lab allows...Plasmids: These plasmids are similar to the Csy4 polycistronic system described above, except that they use...gRNAs are flanked by glycine tRNAs to create polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic...

  22. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...quantity and quality of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires...plasmid copy number   Pro tip! If you are having trouble getting sufficient plasmid DNA, you may need to...factor label Non-silica based, avoids use of chaotropic salts   What are all these other kit types...manufacturers provide specs, protocols, and quality control information, but the level of detail can vary. ...the rationale behind each step and provide troubleshooting advice. In these cases, it can be helpful to... instructions and extensive references for troubleshooting. If you need a high level of consistency between...

  23. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled ...occurring complexes present in the cell, it can also introduce non-physiological RNA-protein interactions due....  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when...when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time point....

  24. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...low-throughput methods such as patch-clamp electrophysiology, a technique that’s used to measure electric...selected for characterization with patch clamp electrophysiology. All thirty were functional and their measured...experiments, neurons expressing ChRgers produced stronger photocurrents at low light intensity than existing...the mice to increase their nose-poking behavior. Strong nose-poking behavior is exactly what was seen with...self-stimulation response. This result showed that ChRger2 stronger photocurrent properties make it better suited ...

  25. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...autophagy. Morphological assays Electron microscopy: Electron microscopy is a traditional method for...this is indicative of protein degradation). To control for protein degradation due to other pathways, ...at various stages of degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged...emit red and green fluorescence. After 4 hours of nitrogen starvation to induce autophagy, red but not green...

  26. All in a Twist: dsRNA

    Type
    Blog Post
    ...If you know the sequence of a virus, you could introduce some of its dsRNA intermediates into a healthy...the inflammatory response associated with the introduction of dsRNA. Synthetic dsRNA Researchers have...time, even before we knew about all the diverse in vitro production mechanisms. siRNA, also known as RNAi...and repetitive regions, such as telomeres and centromeres. These genomic regions are prone to persistent...  Susanna Stroik...

  27. Antibodies 101: Conjugation

    Type
    Blog Post
    ...fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody...capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both of which...modifying the antibody sequence or that rely on strong protein-protein interactions with other peptides...of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides...of antibody conjugation. But hopefully, this introduction provides a good base from which to dive deeper...

  28. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...biology - even a piece of wood - there’s a lot of control spatially as to what’s happening at different points...cellulose fibers, pattern, shape. All of that is controlled by regulatory networks in the plant. If we want... hundreds of genes and regulation and resource control - everything that’s necessary on that scale.  If... terminators. That gives us enough hardware to control hundreds of genes. We’ve created enough regulatory...transcriptional and translational activity a cell has and control how much it taxes the resources of a cell. The ...

  29. Working with Nuclear Receptors

    Type
    Blog Post
    ...reproduction. Both physiological processes are controlled by deliberate and compulsory actions instigated...corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins A and D, thyroid hormone...complex can serve as a rate-limiting conduit to control the actions of the whole complex.  For example,...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators...

  30. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small...less than 10%. In contrast, the original paper introducing prime editing demonstrated ~20-50% efficiency...fold (Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering...ligases to act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime...found that the mutations that emerged depended strongly on the target edit (Doman et al., 2023). Therefore...

  31. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...could provide the basis for student projects in introductory labs. Genome projects use computational methods... of MET/Met genes from the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds...absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation should...PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham...over time: Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1...

  32. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... disease. To do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques... B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark...infect target tissues. Improved specificity through control of gene expression and the selection of specific...CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use...

  33. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Tsang Fluorescent proteins (FPs) traditionally introduced in Caenorhabditis elegans on extrachromosomal...gene into the target gene’s native locus, but introducing large genetic changes in C. elegans with CRISPR... donors (ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split...protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less than 60 nt...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

  34. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...

  35. Hot Plasmids February 2024

    Type
    Blog Post
    ...systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Nature Communications...Peptide-assisted genome editing for primary cells By Susanna Stroik Even with the advances in CRISPR technology over...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection...viral vector has brain endothelial cell-specific tropism, with slightly reduced efficacy compared to AA9...

  36. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...method can be the absence of suitable controls. The best control for this type of assay is a clonal cell...using a reverse transcriptase mutant in a separate control reaction. This will allow you to quantify the amount...of the qPCR target gene. Developing the proper control line typically requires transducing with a limiting.... 3. Zhang, Bing, et al. "The significance of controlled conditions in lentiviral vector titration and...

  37. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... our members took an Arduino microcontroller and a motor controller and made it spin again!  We’ve also...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...instance, the centrifuge in the picture had a broken controller and would not spin. It was going to cost far ...far more than it was worth to repair the controller and a new replacement was not possible, but one of our...

  38. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ... produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation... the reprogramming factors were delivered via retroviral infection [8]. The work was taken forward the...generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ...

  39. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...qPCR assays, samples, standard curves, and normal controls must be run in triplicate.  The nice thing about... of DNA in your sample relative to your normal control - you can set up your calculations so that your...so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either made... does not require a standard curve or a normal control, it can take a few hours longer than qPCR to run...AT-rich DNA and by variation in amplicon length. Am J Trop Med Hyg. 2008 Dec;79(6):887-92. PMID: 19052298. ...

  40. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ... mutated gene. In a Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves...include their episomal nature and very efficient introduction of DNA into therapeutically relevant, non-transformed...B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ...adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016...

  41. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation... cellular level there is a universal theme - uncontrolled cellular proliferation. We have appreciated ...appreciated for some time the causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes...opposite task, keeping cells from dividing out of control. One may compare the roles of proto-oncogenes and...

  42. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...of all distributed plasmids as part of quality control, but does not perform functional tests. The newest...produced from a plasmid in cells. Additionally, in vitro assembly of NgAgo/ssDNA requires incubation at 55...can conveniently transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA...does however rely on cloning into a vector or in vitro synthesis, which could be less desirable if one ...et al. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute." Nature biotechnology ...

  43. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...drug-resistant population compared to a non-treated control group. In this scenario, gRNAs that are “enriched...future therapeutic targets for diseases causing uncontrolled cell growth, such as cancer. Figure 2...As with any experiment, the use of appropriate controls, multiple replicates and several cell types can...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries Find More CRISPR Resources...

  44. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic...life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with...larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting cells of the...epithelium (RPE). AAVs, on the other hand, have broad tropism in the eye. AAV-2, the first identified serotype...

  45. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized sequences...protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag (Figure 3)....unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...interested in neuronal signal transduction, and uses electron cryo-microscopy to understand receptor function...

  46. MXS Chaining

    Type
    Blog Post
    ... create a single polycistronic 15 kb insert. The final construct was then introduced to HeLa cells (Figure...resulting HeLa cells show robust labeling, with strong expression and detection of each fluorophore at...meaning that (in the previous example) one can control whether the third module is added to the 5’ terminus...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  47. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...now many variations, including many that use auxotrophic rescue genes as reporters and some that repress...example of the former. In this system, histidine-auxotrophic host strains are used to select for binding-dependent...accounted for using appropriate replicates and controls (see Tips below), and by validating leads with...reporter gene activation. Including a prey-only control also provides a baseline level of reporter activation...that are known to bind serves as a good positive control to ensure that your setup works. The last thing...

  48. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system ...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...Addgene: Your lab recently developed a retroviral enChIP system using CRISPR technology. Can you describe...Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita...

  49. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...research. Many other mutations have since been introduced to GFP and new iterations of fluorophores are... Transcription Reporter: Placing GFP under the control of a promoter of interest can be used to effectively...Scientists can use light to detect, measure, and control molecular signals, cells, and groups of cells in...Plasmids of this type may have the GFP under the control of an additional promoter from that of the gene...fate studies. It can also be used, when put under control of promoters of interest, to visualize the developmental...

  50. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...organisms to control their movements and behavior. These functions and others are controlled by collections...tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques... et al. "High-performance probes for light and electron microscopy." Nature methods 12.6 (2015): 568. ...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...
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