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  1. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...Addgene recently moved to our brand new location, with more space to grow and help scientists share resources...Gene names! This is not surprising for Addgene. But the names we chose were not just any gene names. Now,...embryonic development The largest conference room at Addgene is named Sonic Hedgehog but this is not a direct...divide properly into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can ...the stomachs of different animals. Check out the Addgene website for plasmids that contain tinman or the...mammalian homolog Nkx2.5. We also have one plasmid, Addgene plasmid 34260, that contains bagpipe from the Deplancke...

  2. Editor's Choice, August 2016

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    Blog Post
    ...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses a newer, CRISPR-based...advice on how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend...

  3. Addgene’s First AAV Data Hub Challenge

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    Blog Post
    ...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  4. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

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    Blog Post
    ... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Professional Organization Resources on Addgene.org Check out Career's at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills of many...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...existing. Here are a few types of teams that exist at Addgene - team types that could exist anywhere in science..., they must work in sync to get tasks done. At Addgene, the Scientist Team is responsible for guiding ...

  6. Antibodies 101: Epitope Tags

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    Blog Post
    .../nature18308   Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide ...applications. You can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well...clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with monoclonal antibodies to Myc...both cleave after the DVPD sequence. Check out Addgene’s monoclonal antibodies (mouse, rabbit, and chicken... antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential tag issues Tags have...Antibody protocols Additional resources on the Addgene blog Antibodies 101: Epitope availability Plasmids...

  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...plasmids (Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...topic page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent...SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene #104106 - #104108) (Figure 3) that can be introduced...PubMed PMID: 24746791. Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs...fluorescent protein collection Find AAVs at Addgene ...

  8. 'Tis the Season to #DeckTheLab

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    Blog Post
    ...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...and tweeting it with the tags #DeckTheLab and @Addgene. We’d love to see how you’re decorating your lab...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

  9. Help Us Name Blugene’s New Virus Friend

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    Blog Post
    ...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search...the finalists will receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new...their virus friend a gender neutral name. The Addgenies will choose up to four finalists and we’ll ask...

  10. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ...how they will use plasmids and viral preps from Addgene to further their work. The Association for Creatine...channel. With the BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional...keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins...Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these candidate genes in ...candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our aims to correct X ... these pathways using biosensors as well. “The Addgene catalog would allow us to enhance our biosensor...upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! Members of...

  11. CRISPRainbow and Genome Visualization

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    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic...PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling...PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of...PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering...

  12. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check out our CRISPR Featured...PMID: 29070703. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. “RNA targeting with...PMID: 28976959. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. "C2c2 is a single-... PMC5127784. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid... PMC5526198. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "RNA Targeting...PMID: 28475872. Find plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase...

  13. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...and tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs...constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research and discovery...from the repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science...constructs for GpNLuc and OgNLuc are available through Addgene (Cat.# 70185 and 70186, respectively). Antares...The construct for Antares is available through Addgene (Cat. # 74279). Enhanced Nano-Lanterns  Suzuki... Nano-Lantern constructs are available through Addgene: The Turquoise (CeNL; Plasmid #85199), Yellow (... explants. This construct is available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki...

  14. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...component available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab...the ID to look up the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid...Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench lab share this...

  15. Hot Plasmids - October 2020

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus...has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral...to cellular glycosylation. Get the library at Addgene CasRx is a RNA targeting Cas system that can be... Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening... with alternative exons. Get the library from Addgene New from the viral service By Jason Nasse We...18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent...

  16. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...data in particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements...

  17. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Learn about Photoactivatable...proteins. To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka...ready-made Nano-lantern biosensors are available from Addgene! References Saito, Kenta, et al. “Luminescent ...: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et al. “Expanded palette of Nano-lanterns...: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, et al. “Five colour variants...Use Rosella to Study Autophagy Resources at Addgene.org Check Out All Our Fluorescent Protein Resources...

  18. Plasmids 101: Inducible Promoters

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    Blog Post
    ... on Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox...Many of these promoter systems are available at Addgene! Chemically inducible promoters Chemically regulated... Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter...the promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible ...allowing reporter gene transcription to take place. Addgene also has popular light-regulated two component ...writing of this article. Additional Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids...

  19. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...plasmids from this publication at Addgene. More Resources at Addgene: Check out more synthetic biology... in other fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor ...PubMed. Find the plasmids from this publication at Addgene. Synthetic biology: engineering Escherichia coli...PubMed. Find the plasmids from this publication at Addgene. A synthetic genetic edge detection program. Tabor...PubMed. Find the plasmids from this publication at Addgene. Multichromatic control of gene expression in ...

  20. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post
    ...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support...

  21. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. A protein-tagging system for signal amplification.... Find the plasmids from this publication at Addgene. ...

  22. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...

  23. Newly Updated AAV Data Hub!

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    Blog Post
    ...experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt!      ... out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share...curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...Submitting to the Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should...step-by-step submission guide or email datahub@addgene.org to learn more. A great submission includes: ...

  24. 2018 #DeckTheLab Contest Winners

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    Blog Post
    ...around the Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating... agar art from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More #agarart from our ...If you’re feeling as chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer ...

  25. Suggest A Plasmid You'd Like to Find at Addgene

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    Blog Post
    ...experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid...Campaign. How to suggest a plasmid The scientists at Addgene work hard to seek out plasmids to add to our collections... potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid ...

  26. 15 Hot Plasmids from 2017

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    Blog Post
    ...our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the latest hot...transposon vectors tested in this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID...spines. The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1...lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat. Methods. 2017. PubMed... and Cre recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed ...check out the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell...Plasmids for CRISPR coselection are available at Addgene, and the Doyon lab has also provided a detailed...

  28. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  29. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  30. Transferable Skills Guide: External Collaborations

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    Blog Post
    ... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate...the community of biological scientists who use Addgene everyday. To get this and other projects like it... it going, someone (likely several people at Addgene) had to take the initiative to find a suitable partner...outside your comfort zone As part of my position at Addgene I am now involved in Strategic Partnership Management...collaborations is important? In my current job at Addgene I work with people from various organizations in...

  31. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...performed on all new viral vector preparations. Addgene has used this method to generally validate our ...   Figure 1: Electron micrograph of an Addgene AAV viral vector preparation after negative staining...    Which titer do people usually report? Addgene and other viral vector manufacturing facilities...compared.   Figure 2: Label that comes on Addgene’s AAV aliquots. Our titer values (circled in red...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because (...AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’re reordering...

  32. Clear and Accessible Writing

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    Blog Post
    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

  33. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...achieve temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts...Want to transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9...on/off promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene...degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron...PubMed PMID: 29581585. Additional resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid...for my Experiment Additional resources on the Addgene website Promoter Guide CRISPR Guide Lentiviral...

  34. Lucky Thirteen: Guest Contributors of 2023

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    Blog Post
    ..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...guest authors, while others chose to work with Addgenie writers to help share their research, tools, and...

  35. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources Find...

  36. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

  37. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post
    ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

  38. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the...supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number ...27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-guided DNA interference...PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the ...PMCID: PMC4446448. Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High...Genome Engineering Posts Additional Resources on Addgene.org Check Out Other Argonaute Technologies from ...

  39. Exploring Applications of the Bioluminescent HiBiT Tag

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    Blog Post
    ...ready to request from Addgene so you can combine the sensitivity of HiBiT with Addgene's quality control, ...Collection that express similar constructs available at Addgene. Read the original paper for more detail on whether...disruptive tag. Find HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors...flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization...Find HiBiT-EGFR and EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors...receptor complex. Find sHiBiT-KDR Fusion Vector at Addgene! HiBiT advances detection of endogenous proteins...

  40. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ... deposited the plasmids used for this study at Addgene which include a C-terminal deletion in the spike...containing these mutations have been deposited at Addgene. Plasmids deposited from Alejandro Balazs’s lab... Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also ...investigate how the virus interacts with human cells. Addgene’s mammalian target plasmids express these and other...Brunello (knock out) and Calabrese (activation), from Addgene to better understand which human genes are important...10.1089/crispr.2020.0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 -... Plasmids & Resources page Stay up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2...

  41. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check Out Our CRISPR Featured... PMC5127784. Find plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic ... PMC5526198. Find plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting...: 28475872.  Find plasmids from this paper at Addgene.  5. Abudayyeh, Omar O., et al. “RNA targeting...PMID: 28976959. Find plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional...Multiplex Genome Editing with Cpf1 Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  42. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View...26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing...27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals...27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary...

  43. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...handful of Click Biology tools available at Addgene. Addgene Link Click Biology Tag Description Humberg...by dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring stable ...signaling. Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding .... Many of these tools are already deposited at Addgene in multiple formats: for use in different organisms...s41589-025-01944-x   Additional Resources on the Addgene Blog Live and Let Dye: Self-Labeling Protein Tags...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...

  44. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...guide to chemogenetics Resources on Addgene.org Check out Addgene's chemogenetics guide Find chemogenetics...and subsequently another round of signaling.    Addgene Tools for Studying GPCR signaling  So with the ...look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate many...GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection...table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.     References and resources...s0014-5793(02)02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide... Various Addgenies...

  45. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1...-HF1. The HypaCas9 plasmids are available from Addgene here! Future possibilities for Cas proteins With...26628643. Find plasmids from this publication at Addgene. 2. Nishimasu, Hiroshi, et al. "Crystal structure...26735016. Find plasmids from this publication at Addgene. 5. Chen, JS, et al. "Enhanced proofreading governs...Browse Our CRISPR 101 Series Resources on the Addgene Website Find CRISPR Plasmids Browse our CRISPR...

  46. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

  47. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...

  48. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

  49. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Addgene’s Top...2011, it was only recently made available through Addgene and we want to highlight it for use in your research...systems have been developed and deposited with Addgene which allow scientists to use CRISPR-Cas technology...determining the structures are now available from Addgene - bfloGFPa1 and bfloGFPc1. Bomati et al., Sci ...further reading about SpyLigase technology, read the Addgene interview with Mark Howarth. Shining a light...ChrimsonR, CsChR, and CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors... of various optogenetics plasmid tools, visit Addgene’s optogenetics guide. More optogenetics tools...

  50. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids and resources page Browse...
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