We narrowed to 835 results for: ANG

... positive change in the scientific enterprise and the way it is funded and to learn tangible skills necessary...necessary for affecting change. On Friday, the symposium includes a panel discussion and keynote by former...the Advocating for Science Symposium Boston 2016 Changes since the Future of Research Symposia Ongoing ...initiated. Recent developments promise to further change the landscape facing all scientists, both young...available avenue. Interestingly, both of these changes share a common thread that has the potential to...for the future No matter your opinion on these changes, it is likely that the shifts caused to the practical...example, implementation and compliance for the FLSA changes, including finding money in already tight lab budgets...

...T7E1, and Sanger sequencing. However, recent studies suggest that both Surveyor™ and Sanger may not be...et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming and cannot...heterogenous populations (Bell et al. 2014). Further, Sanger sequencing has a lower detection limit of 50-20%...Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level...IDLV for in vivo detection (Gabriel et al. 2011, Wang et al. 2015, Osborn et al. 2016) and HTGTS (Frock...magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target mutagenesis ...., et al. "Improving the limit of detection for Sanger sequencing: a comparison of methodologies for KRAS...

...things off, “We are all capable of contributing to change.”  Check out Joanne's Reddit AMA The keynote speaker...spoke eloquently and vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing...PI driven basic research, there is resistance to change, public support is weak, government support is ...Finally, the science publishing system needs to be changed.  Dr. Bourne charged the audience to use their ...engaged in training excellence to begin effecting change.  Panelists call for action Each of the speakers...of how you don’t have to be in academia to make change.  He talked about his own unique way of funding... in the “best” journals.  He pushed for drastic change in the way we train scientists to start addressing...

...introduction of specific sequence changes. To generate a specific sequence change the cell must undergo homology-directed...extensively for the purpose of making sequence-specific changes in human induced pluripotent stem cells (iPSCs)... (ssODN), which contains the intended sequence changes to be incorporated into the edited genome. In mammalian...CRISPR/Cas9 blocking mutations in either the PAM (changing one of the G’s to an A,T or C – but avoid NAG)... to be silent. In our view, it’s always best to change the PAM site, however we have also demonstrated...mismatch in the binding sequence, we recommend changing several bases as close to the PAM as possible ...blocking mutation together with the intended sequence change and screen a few hundred clones to find the right...

... world over. Scientists have traditionally used Sanger sequencing to validate these constructs, as the...relatively low cost and quick turn-around time of Sanger sequencing have historically matched the needs ...and accurate sequencing (“reading”) of DNA, have changed our collective thinking about the feasible size...each sample remain highly normalized across a wide range of input DNA concentrations. This process is explained...plasmids on a plate) will typically have a wide range of input quantities. This variation is problematic...produced from large numbers of samples with a wide range of input concentrations (via proprietary chemistry...differences in input concentration across a 100-fold range (1ng – 100ng) are normalized to two-fold variation...

... Yuan S, Wang Y, Hu B, Yip CC-Y, Tsang JO-L, Huang X, Chai Y, Yang D, Hou Y, Chik KK-H, Zhang X, Fung ...Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z (2020) Characterization...JD, Chu AW-H, Zhou J, Lung DC, Kok K-H, To KK-W, Tsang OT-Y, Chan K-H, Yuen K-Y (2020) Comparative tropism...

...FRCaMPi from the Kiryl Piatkevich Lab (Zhou, Zhu, Eom, Fang, et al., 2025). These new sensors use quite different...jRGECO1a and FRCaMP, FRCaMPi had better dynamic range and sensitivity, in part because its binding affinity...scale bars 50 µm. Reproduced from Zhou, Zhu, Eom, Fang, et al. (2025) under CC BY license. Both groups...10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized red...killswitch" for other biomolecular condensates (Zhang et al., 2025). This new tool offers a way to selectively...leukemia cells, and assembly of adenovirus particles (Zhang et al., 2025). Figure 4: Schematic of the...their dynamics and composition. Reproduced from Zhang et al. (2025) under CC BY license. With 143...

...month project to validate some genomic changes, so this arrangement was ideal for everyone.  The cutting...fit for me, then I didn’t need to explain why I changed jobs. I highly recommend taking on a similar position...major factor held me back; I do not have a second language. During the recession there were many qualified...qualified scientists with experience and a second language in Spanish or French, and so they were ideal candidates...advantage of the fact that science is a global language that allows you to work anywhere and build a network...this company for several years, I was ready for a change.  Working abroad: Research in Australia I then ... fit in with the company and that my ideas and changes were welcomed, which is a benefit over larger companies...

...deaminase. Base changes from C•G-to-T•A are mediated by cytosine base editors (CBEs) and base changes from A•T-to-G...capabilities. Base editors make specific DNA base changes and consist of a catalytically impaired Cas protein...target site, and the deaminase induces the DNA base change without a DNA double-strand break. But base editors...is that the editing domain must induce the base change before the Cas domain lets go of the DNA. ABEs ...hold on” long enough to the DNA to induce a base change. Because of this, ABEs are only weakly compatible... of the fetal hemoglobin gene, ABE8e made both changes simultaneously with 54% efficiency, about 7 times... DNA base swaps, so called point mutations that change C to T, G to A, A to G, and T to C, represent about...

...Other PCR conditions can be changed to further lower fidelity, like changing the metal cofactor and the...This post was written by Alyssa Shepard and Angelo Nicolaci, a PhD student at Moffitt Cancer Center. ...evolution. This is a larger process of gradually changing and selecting the best antibodies for a specific...Directed evolution is a method employed to rapidly change a protein towards a specific goal, like improved... end_module_attribute %}{% end_module_block %} Angelo Nicolaci is a PhD candidate in the lab of Jennifer...23(9), 1126–1136. https://doi.org/10.1038/nbt1142 Kang, B. H., Lax, B. M., & Wittrup, K. D. (2022). Yeast... Hilger, D., Rasmussen, S. G. F., Ring, A. M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display...

...biochemical activity Article contributed by Angela Holmes Jin Zhang’s lab is developing new super-resolution...conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T... to blue light, CRY2 undergoes a conformational change that permits it to bind to the CIB1 (cryptochrome-interacting...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original ...Synthetic decaffeination operon Article contributed by Angela Kaczmarczyk  Listen to the synthetic decaffeination...techniques for live-cell imaging of the dynamic changes in biochemical activity within the cell. Their ...

...References Dong D, Guo M, Wang S, Zhu Y, Wang S, Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis... 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants...10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/35++...//doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah...https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH, Nogales ...

...cutting results in 5’ four base overhangs complementary to the overhangs of the assembled inserts. After...in an orientation that results in 5’ four base overhangs after cutting. The destination vector receives... backgrounds – so much so that there was little range for detectable improvements in the methodology. ...Gate assembly, ligation of mismatched pairs of overhangs can lead to incorrect assemblies, so care must... ligation by this enzyme for all 3- and 4-base overhang sequences under standard reaction conditions. ...identification of mismatch-prone pairings. Using these 4 bp overhang observations, accurate “high-fidelity” junction...can be applied to derive similar high-fidelity overhangs for any Golden Gate assembly design. Number...

... The biggest change is to our most frequently used feature: the search bar. We’ve changed the colors and...Starting today, you may notice some changes to our website! Part of sharing science and making access... development, and content teams have made some changes to the look and feel of addgene.org. Some you’ll...away, while others may be less obvious — but every change is meant to make your experience on our website... on “Deposit Materials” and the search bar will change to an easy-to-use depositing guide, with links ...antibody of the day While those are the biggest changes, there are plenty of less obvious ones to discover...

...PMC2629495. Zingg, B., Chou, X., Zhang, Z., Mesik, L., Liang, F., Tao, H.W., and Zhang, L.I. (2017). AAV-mediated...24694006. PubMed Central PMCID: PMC4137353. Chan, K.Y., Jang, M.J., Yoo, B.B., Greenbaum, A., Ravi, N., Wu, W...PubMed PMID: 18223548. Howard, D.B., Powers, K., Wang, Y., and Harvey, B.K. (2008). Tropism and toxicity....A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., Mortrud, M....Wohnoutka, P., Gerfen, C.R., Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014). A mesoscale...Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). Identification... 195–206. PubMed PMID: 17343566. Tervo, D.G.R., Huang, B.-Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola...

...to iPSCs and re-establishment after Partial rearrangement from one cell type to another Time and resources...vitro as part of the process. This opens up a wide range of possibilities in clinical situations. In cases...19998274. PubMed Central PMCID: PMC2946078. 16. Zhang, D., et al., Highly efficient differentiation of...21151107. PubMed Central PMCID: PMC3033971. 18. Huang, S.X., et al., Efficient generation of lung and ...21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like...2010. 5(1): p. e8763. PubMed PMID: 20098701. 31. Tang, Z.H., et al., Genetic Correction of Induced Pluripotent...PMCID: PMC4835250. 32. Ma, L., Y. Liu, and S.C. Zhang, Directed differentiation of dopamine neurons from...

... Qiao, J., Zang, Y., Gao, Q., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & Wang, X. (2025)...the TAM changes based on the species of origin of the TnpB protein, much like how PAM sites change for different...plasmids. Confirm the correct guide insertion by Sanger sequencing using either 92F or M13R as the sequencing...Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, Y., Nayak, A. K., Baig, M. J., & Molla, K. A. (...

...thanks to our guest blogger, Chinmaya Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the University...post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive...communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto, created...underrepresented minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive...while “manipulate” could mean “worked with” or “changed” to scientists. Keep your message to the main three...Germany in 2017. Apart from The Addictive Brain, Dr. Sadangi is an eLife ambassador, SfN community leader, and...

..., Villiger, L., Zhou, W., Jiang, K., Garushyants, S. K., Roberts, N., Zhang, L., Vakulskas, C. A., Walker...base-to-base conversion in principle, but some changes require multiple steps and enzymes. Prime editing...can encode any of the 12 possible base-to-base changes in a single edit. Prime editing is also more precise...breaks has been widely used to generate precise changes. However, the efficiency of Cas9 cleavage is relatively...al., 2023). Therefore, they decided to evolve a range of specialized prime editors (PEs). PE6a and PE6b...lab! This post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley ...Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure...

... map the turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished...membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox ...respectively, cells will fluoresce either red or orange until recombination occurs. Following the development...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically... to develop a system for tracking the fate of Langerhans cells. In the Ubow mouse, cells expressing YFP...through the proliferation of specific, and not all, Langerhans cells, to create foci of proliferating cells...., Clément, et al. "Multicolor fate mapping of Langerhans cell homeostasis." Journal of Experimental Medicine...