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Showing: 141 - 160 of 1074 results

  1. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult.  ...interaction. Can bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging...cons. Flexible. Can allow people to learn in multiple ways from the same experience.  Can combines cons ...Static. Can be difficult to convey an entire story or context via still images. Some things cannot be photographed...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye or...their audience demographics. Do your research so you can go to where your audience already is.  You’ll also...availability to resources (including the internet!) can vary depending on factors like socioeconomic status...

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    ...key transferable skill that can help you get more of what you want and can also strengthen your relationships...negotiating with my PI on how to spend my grant money (Can I attend this conference?) and with my lab mates ...going on vacation (New York City or Hawaii?). So how can you approach improving this transferable skill? Negotiation...approach to negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all...problem-solving exercise? Asking questions like the below can help you understand the perspective of all persons... person’s request? Your colleague is if someone can feed their cells this weekend - why? Do they have...Someone has to be the first author but another author can present the work at the next big conference - Is ...

  3. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ..., since they can differ. 48” x 36” tends to be the most common poster size, but this can vary as well....readers can navigate your poster and follow the logical progression of your work, and you can accomplish...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...university, you are likely aware of how different posters can be. You have also probably thought about why certain...catch the audience’s eye and draw people in. That can be accomplished with an exciting title and attractive...and potentially a photograph of yourself. A photo can be very useful in the event that you will not be ... your poster at all times, so other participants can identify you. You may also wish to make creative ...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...instance, how much force can be generated by conformational changes in proteins? How can information propagate... R bodies can repeatedly extend and contract in response to changing pH, and they can rupture E. coli...questions can be addressed using R bodies: simple, force-generating protein machines that can extend and...both wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...Papers can be saved to a Semantic Scholar library. You can add hashtags to library entries, which can help...You can also set up alerts for new publications from a particular author. Saving papers Papers can be ...1996, but is getting an update! The latest version can be found here. The new PubMed will become the default...paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following other scientists helps...they support. Monitoring these types of accounts can help you find papers related to a particular topic...papers, so naturally they tweet about papers too. You can follow journals related to your field of research...technically a way to save papers on Twitter, but you can bookmark a tweet in order to easily access it later...

  6. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... parts can be assembled into TUs is key to understanding MoClo. By a similar principle, TUs can be further...many parts can be assembled into many combinations. Even with current technologies, it can be extremely...pair with it can be queried by testing any number in the Level 2 assembly. These pathways can require a ... particular cloning technique. Though rarer, you can find MoClo that relies on Gibson and other non-Golden...cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical levels: individual...terminators, etc. Once selected, individual units can be combined into… Transcription units (TUs), which...composed of multiple parts transcribed together. They can express one or more gene(s), and typically begin ...

  7. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...conference, the audience can help decide which questions get answered. “You can upvote a question and the... you can revisit the Q&A in the chat box. Presenting? Make it interactive! As a presenter, it can be difficult...session from conference to conference can be wildly different. There can be no presenter at their poster, ... “On the flip side, you can attend conferences for just a little bit. You can just go to sessions that... may have apps or interactive calendars that you can use to help plan your conference experience.One of...there was a Q&A section in the interface where you can type in questions for the speaker. “I think a lot...could share their notes together. Figure 1: Can you draw Spongebob from these instructions without...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...titer of a viral vector stock can vary based on transduction target and can also be altered by freeze-thaw... (which can include empty capsids that are devoid of a viral genome). Empty viral capsids can be formed...Different primers can have different annealing efficiencies (Wang et al., 2013). Although you can optimize your...exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more reliable... us information on three important factors. What can AAV titers tell you? Physical titer: the concentration...titer: the concentration of viral particles that can transduce cells. Infectious titers are typically ... after negative staining. Empty vector particles can be identified after negative staining and appear ...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...information can be entered using our online form or by using a downloadable spreadsheet. Either choice can be ...pre-publication plasmids so they can be available when the paper is published. Plasmids can be linked to a peer-reviewed... Strain Addgene can distribute your bacterial strains if they: are E. coli that can be used under BSL1...utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files in any...plasmid cannot be propagated in DH5α. For plasmids with highly repetitive sequences (which can be prone...process. After you have logged in to your account, you can begin the deposit process by clicking "Submit Online... download the .xls file. For the online form you can submit "Published plasmids" or "Unpublished or pre-publication...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...Antibiotic resistance can be scary to think about, but we can all do our part to learn all we can, and minimize...ways in which bacteria can become resistant to antibacterial drugs. Resistance can be intrinsic, meaning...infection before they can test for resistance. Growing and isolating the infecting bacteria can take 24 hours ... for millennia. What we can do, however, is fight smarter, not harder. We can utilize tactics like rotating... is important to note that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and...to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing genes with...methods of ‘horizontal gene transfer’. Resistance can also arise from random mutations in the genetic sequence...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...standard curve can be used to determine the amount of starting material in your sample.  qPCR can also be used...concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify DNA sequences...an in vitro method in which a small amount of DNA can be copied many many times in a short time period.... and essential practice in molecular biology and can be used in a multitude of scientific techniques such...    Steps of PCR The beauty of PCR is that it can amplify DNA using only a short list of reagents and... polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified...denaturing) and cooling (annealing) steps Taq polymerase can copy DNA between the primers using the dNTPs.  Let...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...optimization can be performed to both maximize the separation and reduce any background staining. This can include...clone names, which can allow you to search different providers for the same clone. This can be particularly... process, one must first confirm that a candidate antibody can detect the antigen of interest in the given...proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g. DepMap) – you’ll need...PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your antibody... this post, we will describe the approaches that can be used to determine selectivity of an antibody for...line approach Genetic knockout-based validation can conclusively prove the ability of an antibody to ...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors can exhibit spurious...studying how neurons are physically connected, we can begin to understand how the brain works. It’s kind...roll) neurons! Neurons are really cool because they can transmit information, for example, from one of the...looking at how neurons are physically connected we can begin to understand how the brain works. There are... (Fig. 2). Viruses as neuronal scalpels Viruses can be injected into the brain and efficiently deliver...finding a virus that works well for retrograde tracing can be difficult. The in vivo workhorse adeno associated...rabies) naturally exhibit retrograde transport and can therefore perform retrograde tracing, but often at...

  14. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ask...someone you trust. A good coach can make a big difference, if you can find one. Create your own peer mentoring...if there are other ways they can help you grow. Mentoring relationships can last for an hour or for a year...First, I will describe some of the many ways you can approach finding someone to give you advice and guidance...the training or skills you want to learn. Mentors can be senior to you or peers you admire.  If you see...away We should all have a “posse” of advisors who can help us in different ways and in diverse areas. It... you present or see your work product in action) Can help you learn the local “ropes” and become an insider...

  15. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...2 nm, which can lead to higher resolution for super-resolution microscopy. Nanobodies can be labeled with... the above listed molecular techniques, but they can also be expressed in bacteria and extracted with ... to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes of a primary antibody....multiple labels (green circles in figure 2 below) can be attached to each antibody. But being big is a... target antigen and the fluorescent label, which can reduce the potential image resolution of super-resolution...recognize only one primary antibody. This process can be avoided direct antibody fluorophore conjugates...Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially available...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    ...), which can be fused to recombinant proteins for one-step purification with glutathione, can also protect...instability. Prokaryotes can also have a hard time folding eukaryotic proteins. You can get your bacteria to...ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can maltose-binding protein (MBP...Overexpression can also lead to insolubility, and aggregated protein is not useful protein. MBP tags can help with...biologists who need to overexpress and purify proteins can face any number of technical challenges depending...antibodies available for these tags really are good and can be used for western blots, IP, and affinity purification...unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, ...

  17. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... of the seemingly simple actions we can take to help ourselves can be an appropriate first step. Check...You can work later (in the evening, or in a few years) when life responsibilities are less You can work... a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and tactics that...-don’t list Prioritize! Some things can wait and some things can’t. I kiss my kids good night every evening.... Tell your mom you can’t make it to dinner this week. One swim lesson missed can mean a world of mental...soccer game. Tip: take turns going; that way you can have someone there clapping more often than not. ... your career will suffer. And, yes, your partner CAN take the kids to the doctor without your help. Your...

  18. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s ...immunofluorescence where an antibody can’t be used or that step can be skipped. It has the flexibility ...compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the need...the antibodies used to detect them, and how they can fix your antibody woes.   What are epitope tags?...possible, without the need for an antibody. Tags can enable the separation of detection of a mutant protein...when both are expressed in the same system. Tagging can also aid in protein purification! In this blog, though...s termini to increase detection. The tag can also be removed by treating the protein with enterokinase...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...enzymes that can convert certain chemicals into fragrances that they can smell, or enzymes that can create hydrogels...that students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes ...that they can see, smell, and touch. Along the way, we hope they’ll also see how fun biology can be! What...This is like taking an engine out of the car – you can still use the engine as a power source, but now you...replicating organism. Furthermore, cell free systems can be freeze-dried into pellets (FD-CF pellets), leaving...Most importantly, experiments for FD-CF reactions can be fun and engaging for students. In our BioBits ... hydrogels that they can touch. With BioBits, we want students to make the connection between the facts...

  20. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...neurons, you can see how it contributes to behavior or pathology, and by disabling neurons you can figure out...days. Addgene: Can you tell us a little more about this new two-color tool and what it can enable? Boyden... maybe we can get a handle on some of these mysteries. Another application is clinical; can we find ways...the engineering of its function." Once scientists can do that, they will not only understand how the brain...neuroengineering Addgene: For those who may not be experts, can you give me a brief history of optogenetics? How ... much, much, much more light-sensitive. Then you can use dim blue light and red light together. Understanding... some of the big problems that optogenetic tools can help us to address? Boyden: There are two concrete...
Showing: 141 - 160 of 1074 results