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  1. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

  4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ...

  6. All in a Twist: dsRNA

    Type
    Blog Post
    ...stranded structure produced is known as an R-loop. During transcription, RNA is synthesized from a DNA template...DNA hybrids Persistent RNA:DNA hybrids that arise during transcription are typically recognized by RNase...

  7. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ..., antibiotic resistance, restriction sites used during cloning, and most importantly, the predicted full...ensuring quality, as it will catch any errors made during the cloning, sample handling, or sequence assembly...

  8. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... populations resistant to drug treatments arise during treatment or are preexisting in the parent population... drug treatment and that these are selected for during the treatment process. The authors went on to show...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...same chromosome. Homologous chromosomes recombine during meiosis, creating gametes that contain a mix of...Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor perspectives in biology...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...have errors introduced to the nucleotide sequence during the DNA repair process. The addition of small molecules...to transgenic mice, knockout mice are engineered during the embryonic stem cell stage using one of several...

  11. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    ...QC topics that may require additional attention during analysis. These include topics such as: locations... features. Most of these regions are identified during the QC process, following sequencing and assembly...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome... to survive where others can’t. This ability to endure a harsh environment is distinct from the benefit...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...your sample? Or what if something weird happened during your western and your results look… funky? Do you...remains, you may want to repeat your stripping procedure, perhaps using longer incubation times, or try...

  16. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... competence can arise from recombination events during viral production and poses a biosafety risk to ...While this option is attractive because the same procedure for a single 10 cm2 dish can be easily applied...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...stably integrates into the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi... these plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates...
Showing: 141 - 160 of 441 results