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We narrowed to 753 results for: ELP

Showing: 141 - 160 of 753 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...atlas, including over 3,500 human TF isoforms, to help researchers understand this regulatory network. ...mechanisms, although the unique barcodes for each TF help streamline identification. While the Zhang lab controlled...overexpression programs on cellular processes. For help with library transformation and amplification, refer... yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...

  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy...silico and in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In silico prediction...Treating these cells with the therapeutic antibody can help us assess the effects of the drug on the actual ...in silico method of immunogenicity assessment can help in determining the T-cell epitopes that will likely...

  3. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...finding ways to intentionally set up your bench to help manage details. For example, setting up your pipette...pipette tips to match your plate layout can help you track samples as you add them. Moving your reagents...your left side to your right as you use them can help you remember what's been added and what hasn't. ... as you work, and the possibilities for small, helpful changes are endless.    Fig. 2: Blugene...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ...cells and molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes...system proteins, which can initiate a cascade that helps attack extracellular pathogens.  Transportation ...: IgG1, IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in...isotype heavy chain constant domains. This switching helps B cells adapt during the course of an immune response...

  5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...a selection strategy is not enough on its own to help you find your desired plasmid. In many cases, you...a screen to your cloning strategy? A screen will help you more easily identify successful clones so you...restriction digests to analyze plasmids, which can help you visualize this process, or visit our restriction...just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't be reached...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...CasPEDIA, there is a classification system, CasID, to help facilitate direct comparison of different enzymes...relevant properties of Cas enzymes. That’s a lot of helpful information! To get you started, we’ll give a quick...for a given Cas (SpyCas9a) from CasPEDIA.   Helpful resources and information For some researchers,...delivery of Cas, the size of the protein would be helpful to know for viral packaging limitations. Maybe ...

  7. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...who met every other Thursday for 25 years. They helped one another navigate career changes and overcome...about peer mentoring. They want to find mentors to help them develop their skills and to hold them accountable...brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar direction or...advice. Attend a local networking event together and help each other practice meeting people – compete for...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...cloning experiment. Here are some tips that will help you with your cloning project, and hopefully obtain...possible). There are many softwares out there than can help you at this stage and that can be used to simulate...simulate in silico cloning. This will definitely help in understanding if your strategy will be successful...or human. There are several online tools that can help you with that, they can be open source (i.e. primer3...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...little overwhelming, but here is what I’ve found helpful for my interviews in industry. Prepare stories ...questions. Writing a rough outline of these stories helped me remember them for the interview.  Prepare a ... by looking at their LinkedIn profiles. This can help spark a more natural conversation while also demonstrating...can use notes you took during your interviews to help craft these thank you's. Interviewing remotely Remote...

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...intern. As a member of the Quality Control team, I help make sure the plasmids that go out our doors are...considering my future path. Talking to Addgenies has helped me get a much better idea of what lies ahead. Thus...up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal Komuhendo...gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes in ...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...heard that getting into industry early would only help. Like any major life decision, the transition felt...that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are...varies between companies. Like most jobs, it does help if you know someone who’s already in industry to...Both paths will give you myriad opportunities to help you with the next step in your career.   Many thanks...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...kinds of information on our plasmid pages? Well, to help make the most of these pages, we've written this...plasmid would be useful for their own research, and helps requestors use the plasmid in their own labs. We...additional resources at the bottom of the page that help you cite this publication in your own work: How ...plasmid pages, please don't hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read...

  13. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...those steps and provide you with more details to help you plan and optimize your own protocols. Here we...Antibodies are large proteins that need a little help crossing cell membranes as they are not able to ...permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes...This article on fixation has some more examples to help choose which fixative to use!) Ultimately, the ...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for an inside... we’ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...define for a simple search. These pages provide helpful information about the subject, link to related ...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    ... health and medical organizations are working to help educate the public about proper antibiotic usage...Importantly, native, non-pathogenic bacteria remain to help recolonize the niche, reducing the chance of an ...replication-incompetent phage particles using a helper system. These CRISPR antimicrobials successfully...Future research will show if this new strategy can help us win back ground in the fight against bacterial...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    ...function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can maltose-binding...aggregated protein is not useful protein. MBP tags can help with solubility issues, but scientists may also ...that improve disulfide bond formation in order to help keep your protein soluble.  Tags for affinity and...described in the post, but have been categorized to help you better assess their function. More detailed ...

  17. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...trying to introduce a homozygous mutation, it’s helpful to know how many copies you are looking for. Secondly...untreated population. Software such as CRISPResso can help with the data analysis. If NGS is not an option,...prediction analyses like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing... at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design Tools...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... sequencing can be done, this technique can also help pinpoint the genes within a sequenced genome that...for 1 h at room temperature. After this, it is helpful to subject your membrane to several freeze/thaw...remaining live E. coli. At this point, it can really help to incubate the membranes overnight in 500 mM to...specifically bind your target organism, it can be helpful to spot this protein directly onto nitrocellulose...

  19. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ..., the Antibodies 101 eBook is designed to be a helpful desktop reference. Each chapter brings together...cytometry experiment, “Chapter 7: Sorting” is there to help guide you through the process. If you’re confident.... And, as always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback...
Showing: 141 - 160 of 753 results