We narrowed to 245 results for: GRN

1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   Blog Post

   ...check that your tracrRNA and crRNA (or synthetic gRNA) are derived from the same species. Expanding the...Guide Browse All CRISPR Plasmids Find Validated gRNAs ...

2. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   ... the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion and to target...cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As expected, ...

3. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources ...Check Out Our CRISPR Guide Pages Find Validated gRNAs ...

5. CRISPR 101: Cytosine and Adenine Base Editors

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   Blog Post

   ...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR plasmids for base editing Find validated gRNAs ...

6. The 12 Days of CRISPR: 2021

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   Blog Post

   ... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

7. Twelve Amazing Guest Blog Contributors!

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   Blog Post

   ...software that predicts efficiencies of prime editing gRNAs.   PRIDICT Harvinder Virk and Michael Biddle, ...

8. Performing In Vivo CRISPR Screens Using the FITS Approach

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   Blog Post

   ...types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient...

9. Addgene’s Top 10 Blog Posts from 2014

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   Blog Post

   ...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...

10. Editor's Choice, August 2016

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    Blog Post

    ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

11. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post

    ..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct...

12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

    ... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing...

13. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...

14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post

    ...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...

15. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before...

16. Using Addgene's New Viral Service for Your Research

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    Blog Post

    ...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like...

17. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

    ...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

18. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

19. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...

20. Plasmids 101: Screens vs. Selections

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    Blog Post

    ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...