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We narrowed to 1,144 results for: Gene

Showing: 141 - 160 of 1144 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Blog Posts Prime editing is a “search and replace” gene editing method in which a reverse transcriptase ...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  2. Adeno-associated virus (AAV) Plasmids

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    Collection
    ... based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids ... to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g...Addgene AAV transfer plasmids Viral...disease in humans. For these reasons, AAVs are generally contained at lower biosafety levels and elicit...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...

  3. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post
    ...with the genes in the compacted region and results in a loss of gene expression. In this way, genes in compacted...the corresponding gene products due to the same region, but not the same exact genes, being improperly...? What exactly is epigenetics? Epigenetics is the study of heritable changes in gene expression that do...makes  genes less transcriptionally active. The role of epigenetics in studying disease Epigenetic changes...your genes documentary PBS Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse... change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling...fascinating examples of epigenetics in the form of imprinting. In imprinting, one copy of a gene (either the maternal...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...trying to target every gene in the genome, or a specific class of genes? Addgene currently carries several...within the target gene. CRISPR can also be used to activate or repress target genes without permanently... gRNAs targeting the same gene can be strong evidence that a particular gene is actually important for...2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...which the underlying genetic cause is not entirely known. Identifying which genes are important for a ... experiment is to generate and screen a population of mutant cells to identify genes involved in a particular...Currently, Addgene carries CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2...

  5. CRISPR Activation: A Practical Guide

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    Blog Post
    ...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is under... research CRISPRa for specific gene targets CRISPR activation for genes with known functions has the potential...uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome for example...multiple gene loci simultaneously, CRISPRa can also be used to map molecular pathways and genetic interactions...promoter of another gene, thereby driving aberrant transcription. That being said, we generally pick guides by...constructs allow for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting...potential to be used in therapeutics, as it can increase gene expression at certain loci to create changes in ...

  6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ultrasound...plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive...by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists are ...through a host organism, the expression of some genes can change depending on the location. Current methods...of microbes rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue...ultrasound. These ARG plasmids contain genes for “gas vesicles,” a gas-filled protein structure that water-bound...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit to ...

  7. CRISPR Plasmids - Epigenetics

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    Collection
    ...plasmids for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert Promoter... target a specific promoter or enhancer for your gene of interest. Available modifications include: histone...Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

  8. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...because you optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express...5. Maertens, Barbara, et al. "Gene optimization mechanisms: A multi‐gene study reveals a high success ...getting your genes of interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid...plasmid at Addgene contains a gene that’s been codon optimized for a particular organism, this will sometimes...available from Addgene now have full sequence data, we recommend directly analyzing gene sequences for ...changing conditions - Organisms often need to express genes at different levels under different conditions. ...

  9. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and ...further customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length...versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants...Scientists have worked to optimize this system for gene transfer, studying the stability of modified Ti plasmids...versatile binary Ti vector for both transient and stable gene expression applications in plants. This smaller,...and many plant scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity.... They also replaced the promoter regulating the gene of interest with a constitutive, robust M24 promoter...

  10. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    Blog Post
    ...and easily manipulate specific genes. But what if you want to study genes all across the genome? Two new...7,114 human genes. Unlike the GeCKO library, each library has been enriched for different gene targets. ...designed to target a smaller set of genes and it includes more guides per gene. The Sabatini/Lander library ...screen for genes involved in bacterial toxin resistance, identifying 4 previously unknown genes.  “With ... function of both protein-coding genes as well as non-coding genetic elements.” These lentiviral libraries...library, which they call GeCKO, targets 18,080 human genes with 64,751 unique guide sequences to enable both...including Feng Zhang used the GeCKO library to identify genes essential for cell viability in cancer and pluripotent...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... site (TSS) of a gene of interest, it induces potent gene expression with no genetic alterations observed...efficiently transcribing the gene. This results in gene silencing, with no observed genetic alterations at the ... of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...exploited to either inactivate a gene or introduce a desired genetic alteration. Listen to Our Podcast...need to generate new cell lines or transgenic animals expressing dCas9 as a means of modulating gene expression...Cas9 can be reprogrammed to serve as a regulator of gene expression. Its catalytic domains can be mutated...with a gRNA that directs them to the promoter of a gene of interest, they have been shown to dramatically...

  12. Plasmids 101: Repressible Promoters

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    Blog Post
    ... transcription of a target gene, it therefore determines the timing of gene expression and largely defines...These permit exquisite control of gene expression and tracing of gene expression across development. One...of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101...systems are genetic tools that consist of two parts, both of which must be present for a gene of interest...placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...expression of reporter genes, or to combine reporter expression from one promoter with transgene or siRNA expression...together for complex genetic analysis in Drosophila or for precise control over multiple genes in synthetic systems...

  13. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ... identify genes by their similarities to genes in other species, but these studies generally leave questions... confident that the gene from the test species has a similar function to the gene in the reference species...MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes from...questions about gene function wide open. What if two seemingly similar proteins have acquired divergent...straightforward. Instructors first select a multi-gene pathway to study and a reference organism where ... to answer questions about the evolution of the genes involved in methionine synthesis (5). The budding...strains with overexpression plasmids carrying MET/Met genes from either S. pombe or S. cerevisiae. Students ...

  14. Viral Vectors 101: Viral Applications

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    Blog Post
    ... applications Gene expression control Researchers are always wanting to manipulate gene expression – turn...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...from basic science research all the way to clinical gene therapy. But how can you use these amazing tools... knock it out, etc. It’s how we understand what genes do! Viruses have several options to allow you to... Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily, long-term, and even...engineered to introduce frameshift mutations in genes of interest, turning expression off permanently....

  15. University of Florida Serotype Testing Panel for the Eye and Brain

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    ...vectors for human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 ...cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye...Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090... Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID:...: Viral Vectors As part of our Viral Service , Addgene is producing ready-to-use viral preparations from...preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in...

  16. Plasmids 101: Control Plasmids

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    Blog Post
    ...expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene X The results... level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated...backbone itself on gene expression in your target cells. In experiments employing gene targeting or genome...trying to repress a gene, your control might be a system where expression of that gene is knocked out completely... shRNA targeting human Gene X in Plasmid A is the decreased expression of Gene X. Ergo the positive control...targeting human Gene X. The non-targeting control also assesses any effects of the general introduction ... wherein Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected into human cells...

  17. Plan Your Experiment

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    Collection
    ...mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have a...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific...cloning your gRNAs. Select Genetic Element to be Targeted The exact region of the gene you target will depend...Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells will use error-prone non-homologous...Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without permanently modifying the genome...cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying the genome...Intereference : Target the promoter region of the gene of interest, where the Cas9 protein can block transcription...

  18. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...articles, genes, and blog posts are not comprehensive, so just because you do not see a name or gene on this...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 Keystone...HIST2H2BE RBX1 STAG2 Xist Your favorite gene! SWI/SNF Genes:       BRG1(SMARCA4) BAF170 (SMARCC2...You!     Chromatin-related genes with plasmids available from Addgene The links below pull up lists...containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives new plasmids... In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX ...Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing the Epigenome CUT&RUN...

  19. Zhang Lab CRISPR Page

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    ... information, please visit the Addgene SAM library page . Targeted gene activation using SAM There are...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid... and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV...-Cre recombinase and sgRNAs targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains...

  20. Cancer Research Plasmids and Resources

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    ... RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled... click on a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by ...of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer...
Showing: 141 - 160 of 1144 results