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We narrowed to 310 results for: Gnas

Showing: 141 - 160 of 310 results
  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...
  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’...neuronal networks, and selectively activating signaling pathways. Many scientists came to the Addgene ...
  3. Addgene: The First Twenty Years

    Type
    Blog Post
    ...now-common business practices, like accepting e-signatures and designing a website users could easily navigate...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013...
  4. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...
  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...
  6. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...
  7. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...  Figure 2: Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...
  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...
  9. Career Insights: Technical Support Specialist

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    Blog Post
    ...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...
  10. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...They also host AMA forums where users can ask designated scientists anything about a particular topic....was writing all day and straight on through her designated two hours for answering questions. She was able...
  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance Repetitive sequences Restriction...conservation of codon optimality reveals hidden signatures of cotranslational folding." Nature structural...
Showing: 141 - 160 of 310 results