We narrowed to 181 results for: Lta;
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TypeCollection...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However, if the...the reverse complement oligo. In this case, the resultant oligos would be 24-mer oligos ( Table 4 ). Design...
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Viral Vectors 101: Optogenetic Tools
TypeBlog Post...-activated partners such as light, oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...file. Then I saw it, the chromatogram showing the telltale signs of a mutation introduced by CRISPR. The ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP sites... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...Using in vivo optogenetic stimulations, they simultaneously recorded the electrical impulses of discrete... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...groups (two samples and a control, for example) simultaneously, with up to two independent variables. This... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...strung together, it can read many base pairs simultaneously to increase specificity. This modular approach... -
Genetic Code Expansion
TypeCollection...(AzK) Mammalian TAG Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14 TrpRS-h14 (S8A, V144G, V146C) E. coli... coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9 (S8A, V144S, V146A) E. coli... RF1 function removed Michael Jewett 73581 C321.deltaA (Isaacs lab) all TAG sites changes to UAG, RF1 ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether... -
Antibodies 101: Flow Cytometry
TypeBlog Post...system converts the fluorescence intensity to a voltage pulse, called an event, and assigns each event ... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...expression of multiple proteins on the same cell simultaneously as well as compare between samples. Learn more... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...Allows for visualization of multiple proteins simultaneously with dual labeling when used with two substrates...by ACP Synthase, thereby permitting selective simultaneous labeling in a sample. Please see the note below... -
CRISPR Plasmids - RNA Editing
TypeCollection...destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain RNA editing capabilities... -
MAPK Plasmids
TypeCollection... MAPK-13, PRKM13, SAPK4, p38delta MAPK14 RP1-179N16.5, CSBP, CSBP1, ... -
AAV Viral Preps
TypeCollection...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti... -
Optogenetics Plasmid Collection
TypeCollection...from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then ... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of... -
Brzezinski Lab CRISPR Collection
TypeCollection...A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1 and Vsx2 enhancers in the ... -
Resolute Plasmid Collection
TypeCollection... protein thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement... -
Adenovirus Plasmids
TypeCollection...pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the AdenoBuilder genome...