Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 208 results for: PHI-1

Showing: 141 - 160 of 208 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...expression. Brown et al., Genesis 2014 Jan;52(1):39-48. APEX2 for proteomic mapping and electron...engineering cells that lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of... of human mitogen-activated ERK activating kinase 1 (MEK1) with either one or two phosphoserine residues...comprised of three sets of cloning vectors (Level 0, 1, or 2) which can be utilized in three successive assembly... vectors are directionally assembled into a Level 1 vector creating a single transcriptional unit (Ex:...coding region, and terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 ...vectors to allow for additional iterations of Level 1 assembly if necessary. Combining multiple Level 2 ...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ... Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...expression of firefly luciferase William Kaelin 21375 pHIV-Luciferase Firefly EF1α/IRES Lentiviral expression...stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α Lentiviral expression...

  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...of an industry H-1B need to be aware of the April 1 deadline for companies to submit an application on...and non-profit organizations do not have the April 1 H-1B queue process or quota like industry, these organizations... National Interest Waiver. The three prongs are: (1) The foreign national’s proposed work endeavor must...-1B, you need to prove 3 threshold requirements: (1) that you have international recognition for your ... post may contain technical inaccuracies or typographical errors.  No action should be taken in reliance...

  4. Optogenetics Guide

    Type
    Guide
    ...algae Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific...based on the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester and release proteins...concept of optogenetics. 2012 Prog Brain Res. 196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan...diversifying and extending optogenetics. Cell. 196:1-28. PMID 20303157 Han X, Boyden ES. 2007 Multiple-...Limitations and Future Developments. Exp Physiol. 96(1): 19–25. PMID 20621963 Mattis J, Tye KM, Ferenczi ... in model organisms like mice, zebrafish, and Drosophila. These tools have been instrumental in neurological...

  5. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ... Hot plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for...Ralser's Lab -  Use this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment...

  6. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the passive...sensors in experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse brain slices and freely...Demonstrate DA detection in a single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity...tissues or organs in whole animal models like Drosophila melanogaster, obtaining enough material for a...elements is controlled during development using the Drosophila wing as a model. A typical ChIP-Seq experiment...

  8. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...into three main effector functions.  Figure 1: Fc effector functions in an immune response to a ...interaction between IgE antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation...

  9. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million bp depending on the polymerase...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies,...cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent cells...analyzing the gene’s function. The process is shown graphically in the following cartoon, in which we are adding...

  10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...crevices proteins on the cell surface. Figure 1: Comparison of common antibody, HCab, and nanobody...Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020) Synthetic nanobodies... membranes is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain...

  11. Of Myc and Men

    Type
    Blog Post
    ...more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising monoclonal antibody...genes: deletion mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002...

  12. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing... References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of pLKO.1 TRC-Cloning Vector...information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...marker encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an shRNA...puromycin should be from 1-10 μg/mL in 1 μg/mL increments. d. Label plates from 1-10 and add appropriate...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...you do just this, probably in less than a minute (1). Green Listed is a new software tool used to design...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR...different academic labs, targeting mouse, human, Drosophila melanogaster, and T. gondii are available through...Central PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

  15. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    .... Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research...special category, as has been the ‘labor-of-love’ philosophy of academic science to date. Advocacy opportunities...that the shifts caused to the practical and philosophical landscape of the scientific enterprise will ...University Ray Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology...

  17. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...and the chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...designed to shuttle between species. Figure 1: Shuttle vectors are vectors that can function in ...the vector to survive while the auxotrophic cells die. Some auxotrophic selection markers can be used across...choose to ensure it works in your host species! Auxotrophic selection Another option is to use a different...different method of selection entirely. Auxotrophic selection relies on the host organism being unable to synthesize... example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments...spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing stable mammalian...

  19. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ... genes with a single viral vector.   Fig. 1: Schematic of multiplex gene editing with DAP array... multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes, across four...multiplex genome engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented...

  20. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...red light rapidly deactivates the system. Version 1 of this far-red/red light-responsive system is spread... also be used to engineer new pathways with sophisticated gene expression controls. Most cell types do...light; these two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...
Showing: 141 - 160 of 208 results