Addgene

We narrowed to 227 results for: SIR

Showing: 141 - 160 of 227 results

Results per page:

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

...editors are precise molecular tools, they do have undesired off-target editing effects. ABEs have been described...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

...To utilize HDR, a donor template containing the desired sequence flank by homology arms must be delivered...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...
Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...
Plasmids 101: Modular Cloning

Type

Blog Post

...of a combination of multiple genes to achieve a desired effect. This is commonly used in plant biology ...
CRISPR in the Clinic

Type

Blog Post

...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

... and to work in a variety of applications. The desired specificity of your secondary antibody depends ...
Design Tips for Prime Editing

Type

Blog Post

...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...
A Guide to Designing a Scientific Poster: Content, Layout, and More

Type

Blog Post

... that links readers to more information if they desire it. Shapes. In this type of poster, written and...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

... your own purposes, or delete the originals if desired. It works as a whole or in part. For example, you...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...insertion site, the corresponding gRNA might be undesirable due to potential off-target sites or low predicted...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...investigators to identify specific clones with desirable properties, either initially, or using secondary...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...
Antibodies 101: Flow Compensation

Type

Blog Post

...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...
Plasmids 101: Degron Tags

Type

Blog Post

...advantages over the alternative systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of...
Hot Plasmids: Winter 2025

Type

Blog Post

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...exploited to either inactivate a gene or introduce a desired genetic alteration. Listen to Our Podcast Interview...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

... recombination can occur in multiple ways. The desired recombination event will remove NeoR only and leave...

Showing: 141 - 160 of 227 results

Results per page:

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Mouse Modeling, Part 1: Genetically Engineered Mice

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Corralling and Cornering the Goldilocks Antibody

Plasmids 101: Modular Cloning

CRISPR in the Clinic

Antibodies 101: Choosing the Right Isotype

Design Tips for Prime Editing

Hot Plasmids and Viral Preps - March 2021

A Guide to Designing a Scientific Poster: Content, Layout, and More

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Cloning Mammalian Cells with the Agarose Method

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Antibodies 101: Flow Compensation

Plasmids 101: Degron Tags

Hot Plasmids: Winter 2025

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections