We narrowed to 237 results for: SPL

1. Single-cell tracking of lineage and identity with CellTag

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   ...tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating its...

2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...with a 445/30 nm excitation filter, a 470 nm beamsplitter, and a 495/30 nm emission filter. This combination...

3. Summer SciComm Series: Master of SciComm

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   ...illustrate the breadth of knowledge and passion on display in our building, each day brushing past and chatting...

4. Hot Plasmids and Viral Preps - September 2021

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   ...disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing premature...

5. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ... it recombines at the 5’ and 3’ LTR regions, it splits into two smaller plasmids. The ~3.7 kb recombined...

6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...involved in their growth and extension. Now, they split EB1 into two halves (Fig. 1): one containing the...

7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   ...replication process whereby one cell copies itself and “splits” into two cells. Viral vectors Viruses are quite...

8. What the HEK?

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   ... fibroblast. However, Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent...

9. Identifying Sequence Elements with SnapGene's Feature Database

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   ...ability to annotate a raw plasmid sequence and display frequently used genes and control elements. Development...

10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production (Oyaas,...

11. Hot Plasmids: FRET-Based Biosensors

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    ... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...

12. Learning Technical Skills at a Lab Central Workshop

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    ...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    ...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...

14. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

15. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...

16. The Power Behind NGS Plasmid Validation: seqWell

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    ...because the sample identity information is always displayed at the individual read level, and because custom...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

18. Targeted Mutagenesis with EvolvR

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    ...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

19. Hot Plasmids - May 2022

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    ...work, they have found at least two variants that display increased neuronal expression in the brain while...

20. Antibodies 101: ChIP

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    ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...