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We narrowed to 233 results for: SPL

Showing: 141 - 160 of 233 results

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Single-cell tracking of lineage and identity with CellTag

Type

Blog Post

...tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating its...
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Type

Blog Post

...with a 445/30 nm excitation filter, a 470 nm beamsplitter, and a 495/30 nm emission filter. This combination...
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

Type

Blog Post

...sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production (Oyaas,...
Summer SciComm Series: Master of SciComm

Type

Blog Post

...illustrate the breadth of knowledge and passion on display in our building, each day brushing past and chatting...
Hot Plasmids and Viral Preps - September 2021

Type

Blog Post

...disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing premature...
Three Tips for Preventing Viral Plasmid Recombination in Your Samples

Type

Blog Post

... it recombines at the 5’ and 3’ LTR regions, it splits into two smaller plasmids. The ~3.7 kb recombined...
Viral Vectors 101: What is a Virus (and a Viral Vector)?

Type

Blog Post

...replication process whereby one cell copies itself and “splits” into two cells. Viral vectors Viruses are quite...
What the HEK?

Type

Blog Post

... fibroblast. However, Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent...
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Type

Blog Post

...involved in their growth and extension. Now, they split EB1 into two halves (Fig. 1): one containing the...
Hot Plasmids: FRET-Based Biosensors

Type

Blog Post

... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Type

Blog Post

...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Type

Blog Post

...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...
The Power Behind NGS Plasmid Validation: seqWell

Type

Blog Post

...because the sample identity information is always displayed at the individual read level, and because custom...
Learning Technical Skills at a Lab Central Workshop

Type

Blog Post

...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Type

Blog Post

...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...
Fluorescent Protein Guide: Empty Backbones

Type

Collection

...Expression pBAD/His-B-iRFP - Bacterial Expression iSplit 690 713 5 Dimer pPAS-E - Mammalian Expression pK-GAFm...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...
Targeted Mutagenesis with EvolvR

Type

Blog Post

...its native endonuclease activity degrades the displaced strand. Figure 1: EvolvR consists of ...
Hot Plasmids - May 2022

Type

Blog Post

...work, they have found at least two variants that display increased neuronal expression in the brain while...

Showing: 141 - 160 of 233 results

Results per page:

Single-cell tracking of lineage and identity with CellTag

Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

Summer SciComm Series: Master of SciComm

Hot Plasmids and Viral Preps - September 2021

Three Tips for Preventing Viral Plasmid Recombination in Your Samples

Viral Vectors 101: What is a Virus (and a Viral Vector)?

What the HEK?

Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Hot Plasmids: FRET-Based Biosensors

Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

The Power Behind NGS Plasmid Validation: seqWell

Learning Technical Skills at a Lab Central Workshop

Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Fluorescent Protein Guide: Empty Backbones

Luminescent Imaging with Nano-lanterns

Screening for Successful Genome Editing with Digital PCR

Targeted Mutagenesis with EvolvR

Hot Plasmids - May 2022

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