We narrowed to 402 results for: SUA

...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local...your cup of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some...

...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

...Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S ...Considerations There are a few special exceptions to the usual split-FP options. While the BiFC interaction is ...

...world to detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally...Monoclonal antibodies produced from hybridomas are usually a single antibody recognizing one epitope. (Bottom...

...mismatches usually don’t affect the function of the plasmid.   Confirming the insert We usually confirm ...

... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

...characterized and deposited a variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome...and an epitope tag (ALFA-tag, or At) for easy visualization. A cleavage site for endogenous BACE1 protease...

...students in academia. Getting a drug to the market usually takes over a billion dollars and at least a decade...off as an industry postdoc since your funding is usually separate from a project’s or a team’s, it is possible...

...sure your cells don’t stay out too long. Cells usually have their comfort zone around 37 °C and 5% CO2...to pH. Anything between orange and light orange usually indicates that the cells have grown enough, are...

...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... of use, it is important to note that bacteria usually cannot produce functional multi-domain mammalian...

...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor...developed by Promega as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

...Selecting for seeds that have the tDNA insertions (usually via seed growth on antibiotic-containing media)...from the Dinneny lab that additionally allow for visual selection of transformants based on expression ...

...are smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody...fluorophore conjugates are used, but these are usually more expensive than unconjugated primary antibodies...

...immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue...your sample type. Starting with a protocol that usually works in your system and then optimizing from there...

...responsible for each figure. Others still are more casual with a single presenter summarizing the paper’s...could be solicited via multiple avenues including casual request via email, in person, or instant messaging...