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Showing: 141 - 160 of 410 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively known...optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH system...

  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...delivery to be viral-mediated. Transformations are usually bacterial in nature. All three of these methods...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids...

  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...seminar speakers. My eye was caught by a couple of unusual job openings, although they seemed too far afield... a look at our news editors. A fondness for visualization of science? Look at our graphics and design ...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...working with antibodies.) Conformation changes usually fall into one of two categories: ones induced by...or fold in certain conditions? It can help to visualize an abstract protein during your sample prep process...

  7. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions using... this representation is most easily understood visually. As an example, the curves from our recent amplification...

  9. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...second stain, safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram...classification system is incredibly helpful in visualizing differentiations between bacteria, leading to...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...'m exposed to many scientific communities, but usually I only get to scratch the surface of different ...takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint effort of five ...

  11. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...resume should be about. With a CV, information is usually presented chronologically. With a resume however... an asset,” mention “speaks French.” Employers usually don't spend more than 20-30 seconds on a resume...

  12. Clear and Accessible Writing

    Type
    Blog Post
    ... Alt text is used by screen readers to provide visually impaired visitors with information about images...words to describe the image in a way that helps visually impaired readers receive the same information ...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...digests to analyze plasmids, which can help you visualize this process, or visit our restriction analysis...a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers are especially...

  15. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... person.  In an interview setting, this means persuading an employer that your past work has prepared ...the world to do it.  In the lab, it might mean persuasively articulating why the results you've measured...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... PAM sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer... differences in copy number.  And there are the usual transfection drawbacks: transient expression and...

  19. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... methods of neuronal tracing (Fig. 1): one to visualize neurons that send information to a region (retrograde...retrograde tracer, Fig. 1A), and another to visualize neurons that receive information from a region (anterograde...

  20. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will..., the figure below illustrates how sequence visualization software (we used Snapgene here) can help you...
Showing: 141 - 160 of 410 results