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We narrowed to 447 results for: SUP

Showing: 141 - 160 of 447 results

  3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory DNA sequences...promoters or genes each enhancer is interacting with. Super enhancers are particularly good models for building...simultaneously characterize the ‘social networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... Association (PTA) has been instrumental in supplementing that curriculum. It’s through the PTA that I...Spangler Science The Fab Lab Middle school: supplementing the science curriculum Sharing my expertise ...you want to make sure their STEM education is supplemented. A 2009 study by the NSF showed that among US...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian cells) to collect the cell culture supernatant for further processing. It may be helpful to ...immobilized ligands are incubated with cell culture supernatant containing antibodies. The antibodies bind the...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...

  6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...binding) Google Maps: Enhancers and genome math Suppose that you wish to use Google maps to find all coffee...disrupt these enhancers. A real-world experiment Suppose you run a ChIP-seq experiment (think “GPS for proteins...we all know and love. To understand compaction, suppose you have a piece string that’s 10 meters long. ...

  7. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...but you have definitely reaped the benefits. We support everyone that works with Addgene in some way. For...the development process at Addgene. Diversity supports our innovation Our software development team is...This can lead to confusion as to what the code is supposed to be doing. When this happens, we discuss and...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    ...Customizable to our specific needs Modern and well-supported, so we could focus on our development instead ...encouraged to see that a community of Nextflow supporters existed who had created a shared resource of ...modern architecture of Nextflow included built-in support for containers, such as Docker. If you’ve heard...

  9. How to Be an Excellent Trainee

    Type
    Blog Post
    ...successful mentor relationships, asking for mentor support, and choosing your mentor. What isn’t discussed...mentor learns where you are at and the level of support you need from them, and you can understand the ...Be My Mentor? Finding and Asking for Mentoring Support   ...

  10. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...of the high cell-density they support; however, minimal media supplemented with M9 salts may be preferable...measure of control such as the pLys plasmid, which suppresses basal T7 expression. The pLys plasmid contains...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008). They have...into the endogenous allele of the STAG2 tumor suppressor in human cells (Kim et al, 2016). The Waldman...components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery...

  12. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... is customer support, but there are eight other teams who still retain a customer support element amongst...responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day operations...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, which...additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider...transiently transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence...

  14. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...perturbed by the experiment The loading control is supposed to control for, well, loading errors. When using...A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western blots,”...(2013). Stain-Free total protein staining is a superior loading control to β-actin for Western blots. ...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...play. Photoswitches can be used to activate or suppress secondary signaling pathways with light. This ... closely read the publication, including the supplemental data. You’re most likely to find thorough characterization...spectral and kinetic activation information, in the supplemental data instead of in the main text.  Then, check...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...Enterprise crew fight against a genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie...The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous wolf...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...site-specifically label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, ...the attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop...Xenopus oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...recognition, and DNA cleavage. RNA loading Once supplied with a guide RNA — whether it’s an sgRNA or a ...lab mutated the residues in this loop to produce SuperFi-Cas9, a high-fidelity variant that still retains... University of California, San Francisco, with support from National Institutes of Health R01-GM129325...
Showing: 141 - 160 of 447 results