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We narrowed to 618 results for: Set

Showing: 141 - 160 of 618 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...may generate irreproducible data in a research setting, but these events could have disastrous health ...criteria are not exhaustive. Mismatch tolerance settings are often limited to off-target sites of <4 bp...clinical approval. It is key to maintain a consistent set of standards as the field seeks to generate reproducible...streptavidin and next-generation sequencing (BLESS) (Crosetto et al. 2013). GUIDE-seq and BLESS detect double-stranded...25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    ...summer role was incredibly valuable because it was a set duration of only three months and made a good job... in a developing tropical country. This is why I set up a placement working in a rural health clinic in... can be a glut of individuals with similar skill sets. This is not to say that all commercial companies...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...options depending on above selections) Module set (the set of components you want in your vectors) Target... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection...destination vectors contain an ampicillin resistance cassette and an origin of replication, which are not schematized...

  6. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...published papers that contain that term. You can also set up alerts for new publications from a particular ...similar to Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish... can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly... the work done as quickly as we could. From the outset, we spread out the risk for the project by initially...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... as the overall alignment results. The default setting is “Optimize for Highly similar sequences (megablast... it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are inherently...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene includes...illustrates the utility of PhoCl in a variety of settings. For instance, inserting PhoCl in between a protein...molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the supervision... of Minnesota recently deposited a comprehensive set of plasmids that can be used for either TALEN or ...kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette via Cre...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...the transcription factors from a polycistronic cassette. However, it was unknown whether variations in...

  10. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...PCR machine. Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step...create thousands of identical copies using a simple set of reagents and a basic heating and cooling (denaturing...choosing primers. Place thin-walled PCR tubes on ice Set up a 50 μL reaction (Keep all your reagents on ice...designed to have similar melting temperatures (Tm). Set the annealing temperature to 5°C lower than the Tm...MgCl 2 and/or 1µl DMSO to each reaction. The ideal setup for this troubleshooting step is to do one reaction...

  14. Transferable Skills: Negotiation

    Type
    Blog Post
    ...variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting that researchers...professional negotiation look like? In professional settings, creative negotiation skills are key to maintaining...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...

  15. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...applications. These PCR methods all use the same basic PCR set up and steps but differ in how the PCR products are...amplified using several forward and reverse primer sets.  Multiple template PCR - multiple templates with...

  16. PhD Applications After COVID

    Type
    Blog Post
    ... of the environment of the department, the lab setting, and how students truly feel about the program ...stressful time, and I was more relaxed during both sets of interviews. I also had universities change their...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...have skill in a particular technique? Do you have a set of tips you give to every new lab mate? Do you have...

  19. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...!). Then schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    ...way to develop and exercise your scientist skill set. If you dread the thought of writing a review, or...I was by no means an expert on this topic when I set out to write the review. That’s just to say that ... isn’t possible. So, define your scope from the onset. Perhaps you, a colleague, or your adviser was invited...
Showing: 141 - 160 of 618 results