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...options depending on above selections) Module set (the set of components you want in your vectors) Target... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection...destination vectors contain an ampicillin resistance cassette and an origin of replication, which are not schematized...

...published papers that contain that term. You can also set up alerts for new publications from a particular ...similar to Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter...

...well-studied and inexpensive. In addition, a complete set of well-annotated deletion strains is available (...content knowledge and research skills (8). A complete set of student and instructor resources is available ...

...polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less. The ligations combine...allows one to simultaneously complete an ordered set of scarless ligations following digestion with Type...

...follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team to accomplish... can detect the virus in a clinically relevant setting. Molecular diagnostics for the Zika virus predominantly... the work done as quickly as we could. From the outset, we spread out the risk for the project by initially...

... as the overall alignment results. The default setting is “Optimize for Highly similar sequences (megablast... it saves analysis time to remove this default setting. Timesaving Tip #2: blastx searches are inherently...

...structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene includes...illustrates the utility of PhoCl in a variety of settings. For instance, inserting PhoCl in between a protein...molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under the supervision... of Minnesota recently deposited a comprehensive set of plasmids that can be used for either TALEN or ...kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette via Cre...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...the transcription factors from a polycistronic cassette. However, it was unknown whether variations in...

...PCR machine. Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step...create thousands of identical copies using a simple set of reagents and a basic heating and cooling (denaturing...choosing primers. Place thin-walled PCR tubes on ice Set up a 50 μL reaction (Keep all your reagents on ice...designed to have similar melting temperatures (Tm). Set the annealing temperature to 5°C lower than the Tm...MgCl 2 and/or 1µl DMSO to each reaction. The ideal setup for this troubleshooting step is to do one reaction...

...technology Here’s some advice on how to successfully set up your qPCR to determine the genome copies in a ...

... of the environment of the department, the lab setting, and how students truly feel about the program ...stressful time, and I was more relaxed during both sets of interviews. I also had universities change their...

...variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting that researchers...professional negotiation look like? In professional settings, creative negotiation skills are key to maintaining...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...

...applications. These PCR methods all use the same basic PCR set up and steps but differ in how the PCR products are...amplified using several forward and reverse primer sets.  Multiple template PCR - multiple templates with...

...of wild-type TadA and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess...from the complex the researchers developed a second set: The ABE8.x-d version carries a fusion of wild-type...

...one set of genetic loci can be introduced together with truncated gRNAs targeting a different set of loci...

...proposing new programs, we're always striving to set an example of environmental stewardship.  Additional...

...have skill in a particular technique? Do you have a set of tips you give to every new lab mate? Do you have...

... you started using CRISPR in your research and setting up CRISPR experiments. We will focus on making ...DNA to serve as template for the repair. Once you settle on your gRNA, you can design a donor DNA to have...variety of modifications and will likely contain a subset of wild type cells. If you are screening a variety...

... and their team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent...response. Evolution of a new variant Maria and the team set up a directed evolution and screening experiment ...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...!). Then schedule a meeting to discuss the work, set expectations, answer any questions, and (most importantly...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...doesn’t end after you get your first new job. To set yourself up for success in the career you hope to...