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We narrowed to 617 results for: TIL;

Showing: 141 - 160 of 617 results

  1. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...limitation. The procedure will also need to be perfected until the efficiency of repair approaches 100 percent.... fundamental questions about CRISPR/Cas biology still left to answer as well, she says, and those details...increasing effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of ...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ...requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because of its lowered editing...coexpressed proteins run as ladders. Image from Santilli, et al. 2021 Plasmids are used to express ...via engineered immunoglobulin binding domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...community-driven life of its own. Nevertheless, there are still many open questions to be clarified, especially ... put their work on the shoulders of giants. The still relatively young movement of sharing open hardware...Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression strategies...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

  5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...however, have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure ...located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains.... Excitation of neurons deeper in the brain may still require fiber-optic cannula implantation, but altering...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...time point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli...

  7. dTAG - You're it!

    Type
    Blog Post
    ..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...genetic approaches. In addition, the dTAG system is versatile and can be used in both cell-based assays and ...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...done significant work to improve the Adv genome’s utility in research and its therapeutic potential. Note...use of this system. New and improved systems are still being developed. A detailed protocol for production...should be used as an internal control (ATCC#VR-1516) until the assay is validated. Infectious titer: (Plaque...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR...and its role in gene expression, though this may still unexpectedly affect nearby genes (Figure 1A-C). ...lncRNAs. The Cas14 variant is a smaller and more versatile option. It can be used to target lncRNAs with ...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...identifying a suitable route of administration. However, until recently safe delivery of nucleic acid cargo (DNA... of blindness and was thought to be untreatable until 2008. Following a single dose of subretinal administration... and other ongoing clinical trials support the utility of AAV as a broadly applicable gene delivery tool...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...focus on one such strategy--the FLEx switch--which utilizes recombination elements to turn off expression ...introduced, recombination can proceed either by first utilizing the loxP sites or the lox551 sites. Regardless...reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided. Since...

  12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...” identified seven types of difficult people: Hostile-aggressives, who are usually antagonistic and impolite...is a difficult person, the above strategies will still be helpful but you will need to be more assertive... try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all experts, ...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...done the technical design of your blot, but are still finding yourself having issues, you’re in the right...blocking conditions on the spec sheet. If you’re still seeing high background after following manufacturer...yourself. But even with a validated antibody, it can still take time for you to get it to work well. Dilutions...antibody dilution. Adjust the antibody dilution until your protein dilutions are in the dynamic linear...

  14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...frequency. Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter ...editing; for known promiscuous gRNAs, xCas9 3.7 still showed a 4.2-9.4-fold improvement in off-target:...these early data are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9...

  15. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...after a semester while the other student stayed until he graduated. Years later I attended a formal training...team members by giving them new opportunities. I still need to check myself sometimes to make sure I am.... In many cases reports are not aware of issues until you bring them up. I wouldn’t go as far as saying...

  16. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as a small molecule...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...the pandemic; for more discussion on that, see Pyhtila et al 2023.)  One of our favorite observations ...CRISPR Plasmids by Addgene,” in The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou...publications.  References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. ...

  18. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers can conveniently...unhappy with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their...complex with a 5’-P-ssDNA guide at room temperature. Until that time, those who have been lucky with NgAgo ...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    ... the base of the stem. This buys you a few days until new inflorescences begin to grow. This will also...too old to transform. This transformation could still work, but the efficiency will be low. Tips: Use...plants: If you transform early, your plants will still have plenty of time to produce seeds after you expose...
Showing: 141 - 160 of 617 results