...preparation from the inventory below. Lentiviruses are distributed as purified viral particles in solution that ...

...most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...

...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive...

...subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 ...

...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( ...

...encode a wide range of gene products. The main restrictions on a lentiviral transfer plasmid are size (i.e...

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...

... is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids...

... Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations in engineered...

... final product should be translated as a single string of amino acids that preserves the sequence...

...Nature . 520(7546):186-91. PMID: 25830891 Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts...

... antibody manufacturer for wash recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline...abundant protein if you’re stripping, since you’ll lose protein with every strip cycle. Troubleshooting the...Increase volume of wash buffer Incomplete stripping (If stripping/reprobing) Check for antibody signaling...signaling after each round of stripping* Use a harsher stripping buffer Too little signal Large proteins...Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot...need such precise measurements, you can use a colorimetric ladder, visible to the naked eye; a fluorescent... choose from.  Transfer Transfers are a little trickier than gels and may require multiple runs to optimize...

...e0124958. LOC8-ing mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University...cumbersome. To overcome many of the limitations intrinsic to DNA-guided systems such as TAL effectors or...modified to use different antibiotic resistances, restriction enzymes and plasmid backbones. pUCXKT contains...gene-specific forward primer containing the desired restriction site (the MCS has several options) and a pUCX-specific...resistance cassette, a ribosome binding site, SacI restriction site and a linker region. The PCR product is ...BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round of translation...the protein product. The system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off...

...antibody. TRIM21, an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits...endogenous TRIM21 is sufficient for TRIM-Away, and you only need to deliver the target antibody. TRIM-Away ...Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing... proteasome function. TRIM-away compared favorably to drug inhibition, with TRIM-away targeting of mTOR.... To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding that...changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not aberrantly ... methods for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the...

...journey. Figure 2: Annual distribution of items to countries with a distributor. Arrows indicate the year...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import...country to country.   Figure 1: Number of countries Addgene distributes to per year, 2005-2023. We identified... we identified countries that would benefit from a third-party “helper”: a distributor who, among other... India, and Mexico. Table 2: Addgene distributors Distributor Partnership Start Date Country Served...in material distribution after we partnered with them (Figure 2). In others, the distributor helped solve...that we could identify the need for distributors in some countries. ‌We’ve developed systems to allow ...

...a sequence recognized by the restriction enzyme MstII. When a restriction digest with MstII and southern...than real-time PCR (4). Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence...different restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes...has changed, restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand...This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University...three historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...

...categories: parametric and non-parametric. In this blog we will be discussing parametric tests. Parametric tests...be met for a parametric test are as follows:   Parametric test assumptions: Distribution – Normal Variables...   Fig. 2: Examples of data distribution profiles.   Parametric tests  Statistical tests fall into...derived from a theoretical distribution. If your data is normally distributed, it will form (more or less... If your data does not fit parametric assumptions, then a non-parametric test is a better option. Stay...numerical risk allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve...data doesn’t fit the requirements above, a non-parametric test may be the way to go, which we will discuss...

...top ten countries for each group (Figure 4).    Figure 4: Addgene deposits and distribution by country...deposited by each country. Countries shown are top 10 depositing countries. (B) Total CRISPR plasmids ...requested by each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea...in ~40 different countries, and we’ve shipped CRISPR plasmids to labs in ~100 countries.  The impact Addgene...repository has grown to include many CRISPR tools.  Distributing and depositing In Figure 1, you can see cumulative...cumulative numbers of CRISPR plasmid distribution and deposits (Figures 1A and 1B); the cumulative number...cumulative number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection...

...early-phase clinical trials and have shown promising preliminary results (Dimitri et al). CRISPR engineering...editor-based clinical trial for SCD in 2022 and Editas Medicine starting a Cas12-based clinical trial for SCD in ... This ongoing trial is sponsored by Excision Biotherapeutics. Additional ongoing trials The CRISPR therapies...approved or have made significant progress in clinical trials.     Figure 1: Types of delivery and editing...of the cancer-fighting component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both... returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was sponsored...and CRISPR Therapeutics. Data shared from these trials has been outstanding, with almost all patients ...

...assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...discussed in our restriction cloning post. However, even more than with standard restriction cloning, it is...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...itself are very similar to those outlined in our restriction cloning post with a few quirks specific to the...amplified (usually 18-21bp) When selecting restriction sites, you should use a DNA analysis tool, such...Sequence Analyzer, to allow you to identify which restriction sites are present in a given sequence. You want...