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We narrowed to 398 results for: aga

Showing: 141 - 160 of 398 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... turned to characterizing its cleavage pattern. Again, they were in for a surprise! Whereas Cas9 generates... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR. This “second chance” mechanism...

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    ...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes...Good for purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal replication and balance population growth against sustainability. Plasmid DNA replication and regulation...proteins. In nature, such plasmids ensure their propagation by limiting their copy number and compensating...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...

  5. Viral Production at Addgene

    Type
    Blog Post
    ...possible, all plasmids used for viral production are propagated in the endA-mutated NEB Stable strain of E. coli...plasmid in the viral preparation by performing PCR against unique regions of the transfer plasmid.   Lentivirus...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How...What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial...

  7. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... code, we may need additional clarification and again we collaborate with the Product Team who in turn...would be like re-inventing the wheel over and over again for every single software today that prints labels...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...giving you a plethora of opportunities to explore! Again, attending one of these fairs is a great start, ...sure to get that person’s contact information and again, make a personal connection! Even if you don’t get...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...

  11. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next to these naturally occurring co-factor...biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...PCR machines/Heat blocks, Lateral flow strips, Agarose gel equipment, Fluorescent emission reader Approach.... They also tried to confirm target binding on agarose gels where a cleavage reaction upon proper binding...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...visualize their target. These antibodies are generated against a specific antigen and then conjugated with a fluorescent...that you will be able to image them in the end. Again, the methods you use will depend on target and sample...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...would make it easier for an “escape” virus to propagate and infect adjacent cells. It will be important...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...0059-1 Veres A, Gosis BS, Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, ...

  18. A Control for All Seasons

    Type
    Blog Post
    ... experimental (?) groups were run on a western against a protein standard (P) to check for tau molecular...antibody specificity is to test the primary antibody against wild-type and knockout samples. In this test, wild-type...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light-driven pumps that pass ions (Cl-, H+, Na+) against the concentration gradient and hyperpolarize the...activation inhibitory (ArCH) proton pump passing ions against the concentration gradient. Created with BioRender.com...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ....2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & ...Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates...
Showing: 141 - 160 of 398 results