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We narrowed to 581 results for: arl

Showing: 141 - 160 of 581 results

  2. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...institutions doing after the injunction? We are particularly interested in whether institutions planning ...postdocs at other institutions to see if we can similarly convince them to go ahead with raises. What will...salaries. In addition, salary uncertainty may be particularly problematic for two-postdoc households. To better...

  3. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ... in the final construct, allowing for precise, scarless cloning. The cloning scheme is as follows: the...but Golden Gate assembly can be designed to be scarless. Golden Gate assembly is also less expensive than...Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas groups described ...

  5. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...identical and all mice of the strain are homozygous at nearly all loci. This reduces variability in genotype ...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...Examples of outbred strains include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss...

  6. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...Sainsbury Laboratory. Are you a science student or early career researcher looking to break into science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

  7. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ... scientists to larger groups of trainees at an earlier point in their careers? A CURE for Biochemistry...protein with easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...me). While things were incredibly busy in those early years, the founders knew that company culture and...throwing fun into the mix. Thus, our founders and early employees spent this remaining time by elaborately... you are planning, dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... cell types depending on their serotype, are particularly challenging. Their efficacy in any given system...from 2016–2023. All three generations are regularly requested and a valuable part of our viral vector..., new partnerships, and new platforms, from our early engagement with UPenn to our launch of the redesigned...

  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ...

  12. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...scientific expertise. Organizing a career fair for early-career scientists provided a crash course in this...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

  13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...could produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed...applicability. They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background...next-generation bARGs that could scatter ultrasound nonlinearly and be easily expressed heterologously by bacteria...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... interfering with downstream assays. This is particularly advantageous if the antibody used for IP is ...despite the size difference. Magnetic beads are particularly advantageous because they do not require centrifugation...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...CRISPRa) are great ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...being discarded and replaced with a new vial of early passage cells. Approximately two weeks post-thaw...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...products may be toxic to the host when expressed, particularly those that are insoluble, act on DNA, or are... can neither restrict nor methylate DNA. dcm: Similarly, strains with this mutation are unable to methylate...dead cells, and, conversely, you cannot induce too early as there are not enough cells in the culture to ...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...research directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA...MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various...brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ...transparent. Thus scientists can easily observe early developmental stages which can be difficult in other... large experiments over multiple generations. Similarly to other model organisms, the C.elegans genome... PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease models in Drosophila...

  20. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...fluorescent and can be challenging to track intracellularly, but aptamers permit the study of intracellular...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ...binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach, but binds with much higher...
Showing: 141 - 160 of 581 results