We narrowed to 315 results for: bon;

1. Visualizing Translation at the Single Molecule Level

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   ...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   ...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....

3. Fluorescent Proteins 101: Luciferases

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   ...different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety of expression...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...communication skillsClear communication is the backbone of effective management. Miscommunication often...

5. AAV Q&A with Tim Miles

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   ... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...

6. Antibodies 101: Isotypes

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   ...isotypes, such as the number and location of disulfide bonds, the number of constant domains, and the hinge region...

7. Viral Vectors 101: Viral Vector Elements

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   ...plasmids for their production. As such, there are bonus components to these vectors that aren’t reflected...

8. 15 Years of Addgene: The Top 15 Plasmids

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   ...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

10. Luminescent Imaging with Nano-lanterns

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    ...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags  ...

11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...

12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References...

13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    ...disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when...

14. Troubleshooting Your Plasmid Cloning Experiment

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    ...try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio...

15. Plasmid Tools for Microbiome Studies

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    ...toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin...

16. Summer SciComm Series: Cognitive Load

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    ...students PCR, they first had to understand hydrogen bonds and then build up from there. In every project, ...

17. Antibodies 101: Affinity Reagents

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    ...targets. Due to their size and lack of disulfide bonds, monobodies can be easily expressed in transfected...

18. What's New in CRISPR - November 2021

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    ...various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical...

19. Antibodies 101: Conjugation

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    ...cysteines, such as those that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom...