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  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...deletions and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that...comprises an active Cas9 fused to the reverse transcriptase (RT), which induces two double-strand breaks...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...for viral titration consider using a reverse transcriptase mutant in a separate control reaction. This ...results. A packing vector deficient in reverse transcriptase will produce low levels of virus and can be ...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of this process...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by qPCR...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime editing guide RNA (pegRNA...containing a primer binding site and a reverse transcription template (RTT). The design of the pegRNA has...

  11. Pooled Library Amplifications

    Type
    Blog Post
    ...library has inserts of different sizes (such as a transcription factor overexpression library) or is prone to...libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening, a protocol paper by the...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...useful to me? Plasmid purpose: This is a brief description of what the plasmid was designed and used for...order the plasmid, how will it be sent to me? Description: This describes how the plasmid or other product...

  15. MXS Chaining

    Type
    Blog Post
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  17. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

  18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...functions such as epigenetic regulation and transcription by using locus-specific chromatin immunoprecipitation...biochemical analysis of genome functions such as transcription and epigenetic regulation. To elucidate molecular...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...start by looking at the requirements in the job description. For each requirement, ask yourself if you have...the university]” Include keywords from the job description to help the hiring manager quickly make the connection...
Showing: 141 - 160 of 437 results