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We narrowed to 344 results for: dre

Showing: 141 - 160 of 344 results
  1. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...short-read and Sanger sequencing are limited to hundreds of base pairs, long-read sequencing can read sequence...silico variations and lack of verification. To address this issue, the QC team developed a separate script...
  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal.... Bahi, Amine, Vijay Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of ...
  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...expression in the central nervous system (CNS). To address this technological gap, a trio of recent papers...isoform-specific expression of Bdnf resulted in hundreds of differentially expressed genes between Bdnf...
  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...above, plan to give your 10 minute overview and address the aims in more depth in the remaining time. The...shortage." What is the knowledge gap you plan to address and why is it significant? What is the problem ...
  5. Lab to Office Culture Shock

    Type
    Blog Post
    ...in finance, and Wu in graphic design. You could dread these interactions, but you could also embrace them...happen eventually. If you approach that transition dreading the change, the adjustment period will be that...
  6. Bioinformatics at Addgene

    Type
    Blog Post
    ... between Seqera’s mission and our own, as both address the need to accelerate science using different ...necessary to keep up with our sequencing instrument (hundreds of plasmids each week!), we already planned to...
  7. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and published...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...
  8. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...gene which could affect translation initiation (Dresios et al., 2006) and mRNA stability (Hausser et al...18: . https://doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell SA, Zhou W, Mauro VP (2005) An mRNA-rRNA...
  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...develop and exercise your scientist skill set. If you dread the thought of writing a review, or if you’re currently... crazy (meaning you are probably going to cite hundreds of references) and it’s better to keep your references...
  10. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...usually not enough. How do you stand out from the hundreds of others that did the same thing? Make an effort...relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible position to ...
  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... 35.4 (2003). 812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination of...75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C., Drexler, H.G. “Detection of mycoplasma contaminations.”...
  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...306.5700 (2004): 1370-1373. PubMed PMID:15550670. 7.Andresen, Martin, et al. "Photoswitchable fluorescent proteins...2008): 1035-1040. PubMed PMID:18724362. 8.Stiel, Andre C., et al. "Generation of monomeric reversibly switchable...
  13. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...wave that only excites fluorophores within a few hundred nanometers of the glass/water interface. Super ...used. TIRF only excites fluorophores within a few hundred nanometers of the coverslip (4). Essentially, an...
  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... constructs (Martella et al., 2017). If you can dream up an experiment, MoClo can help you achieve it!...://doi.org/10.1101/2024.02.16.580396. Martella, Andrea, et al. “EMMA: An Extensible Mammalian Modular ...
  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...assay to make sure it is working? This article addresses these questions, which are critical in setting...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop a successful...
  16. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...plasmids in a pooled library can range from a few hundred to millions. Pooled libraries must be verified ...Investigator (PI), provide us with your shipping address for deposit materials, and agree to our terms of...
  17. CRISPR Guide

    Type
    Collection
    ...locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites throughout the chromosome...
  18. Chemogenetics Guide

    Type
    Guide
    ... than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs... protein that the receptors couple to. Gq-DREADDs Gq-DREADDs signal through the Gαq/11 G-protein and activate...with hM3Dq being the most widely used. Gi-DREADDs Gi-DREADDs signal through the Gαi/o G-protein and inhibit...currently two Gi DREADDs based on human muscarinic receptors, hM2Di and hM4Di, and a Gi DREADD based on the...κ-opoid receptor, termed KORD (or KORDi). Gs-DREADDs Gs-DREADDs signal through the Gαs G-protein and activate...turkey erythrocyte β-adrenergic receptor with a rat M3 muscarinic receptor. This DREADD was shown to have...used in vivo. Figure 2: DREADDs, their ligands, and signaling properties. DREADD Ligands Muscarinic-receptor...
Showing: 141 - 160 of 344 results