We narrowed to 369 results for: epor

1. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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   Blog Post

   ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...

2. 25 Work From Home Tips for Scientists

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   Blog Post

   ...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...

3. Genomic Deletions in Mammalian Cell Lines

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   ...transfection conditions for each cell line with a reporter construct to ensure robust plasmid delivery before...

4. Why and how to start studying chromatin (with Addgene's help)

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   ...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

5. Plasmids 101: Inducible Promoters

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   Blog Post

   ... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

6. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   ...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary...

7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID...

8. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ...

9. New and Upcoming Viral Vectors - May 2020

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   Blog Post

   ... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

10. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post

    ... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

11. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...

12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030...

13. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...

14. The Future of Research Symposium Boston 2015

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    Blog Post

    ...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...

15. Working with Nuclear Receptors

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    Blog Post

    ...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol...

16. Allen Institute for Brain Science AAV Enhancer Collection

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    ...expression from mouse to primate neocortex. Cell Reports , 34 (13), 108754. https://doi.org/10.1016/j.celrep...

17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

18. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed ...introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered...

19. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...expression of the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP ...expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where...

20. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo...