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Showing: 141 - 160 of 610 results
  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...
  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...
  3. Plasmid Collections

    Type
    Collection
    ...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections...
  4. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...
  5. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...
  6. TALEN Engineering

    Type
    Collection
    ...yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish ( Sander...
  7. Common Injection Routes in Mice

    Type
    Blog Post
    ... available in your lab or organization, and approvals from the organization overseeing your work. Good...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering...
  8. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...
  9. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources...
  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...
  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...questions, panelists and participants of the symposium began providing means to answer some of these questions...
  12. Synthetic Biology - Overview

    Type
    Collection
    ...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...
  13. Adenovirus Plasmids

    Type
    Collection
    ...adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids...
  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...Management Skills Volunteering at a Professional Organization Resources on Addgene.org Check out Career'...
  15. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption...
  16. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...
Showing: 141 - 160 of 610 results