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...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific...

...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...

...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...excitable cells of the nematode Caenorhabditis elegans. They have found several voltage biosensors and...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...may be due to different lipid compositions of C. elegans membranes, which might beneficially influence the...

...showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...

..., if you are studying a protein that may bind a ligand during your experiment, then you’d want to make...antibody that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody...part of your protein that may be masked while the ligand is bound, then you’ll get a false negative signal...Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological ...

... of informatics. I am a molecular biologist who began coding for my thesis project a few years ago when...language favored by biologists. Almost as soon as I began, I realized that I needed a system to keep track... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...

...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

... Exchangeable HaloTag Ligands HaloTag Reversible binding of fluorescent ligands to HaloTag PCV-Cas9,... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...Addgene in multiple formats: for use in different organisms, targeted to particular compartments, or fused...

...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA...angiogenesis (Witz et al., 2009). Then, scientists began focusing on the extracellular matrix and fibroblasts..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...

...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...

...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...here at Addgene! Along with other open-science organizations, we support scientists in sharing their results...

...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...

...interactions New auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service...Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly of multiple... the auxin inducible degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron...degron (AID) system has been extensively used in C. elegans since 2015 to conditionally degrade proteins of...

...and optogenetic tools exist, humans (like many organisms) are opaque, which makes most biomedical applications...There is a variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021...sometimes seen controversy, and the interactions of organic molecules like proteins with magnetic fields generally...magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011...

...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...predictable kinetics over 14 hours of expression in C. elegans embryos. However, this initial timer was a tetramer...could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. Of course, timers may ...

...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...

...In April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric... amassed in places around the world, hypotheses began to develop that this illness was caused by either....73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & ...00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide epidemiology...