Skip to main content
Addgene
Showing: 141 - 160 of 267 results

  3. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...

  6. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...22222-AAV1 AAV-FLEX-Arch-GFP Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP (PV2527) Ed Boyden AV...pAAV-CamKII-ArchT-GFP (PV2527) Ed Boyden AV-5-PV3446 59170-AAV5 pAAV-Syn-Chronos-GFP Ed Boyden AV-5-PV3447...pAAV-hsyn-Jaws-KGC-GFP-ER2 Ed Boyden AV-8-PV3638 65014-AAV8 pAAV-hsyn-Jaws-KGC-GFP-ER2 Ed Boyden AV-9-...22222-AAV9 AAV-FLEX-Arch-GFP Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527) Ed Boyden AV...29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446 59170...59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger...AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3447 59171-AAV1 pAAV-Syn-ChrimsonR-tdT Ed...

  7. TALENs for the EGFP Reporter Gene

    Type
    Collection
    ...Half-Site eGFP-TALEN-01-Left TAL2000 TCGCCACCAT eGFP-TALEN-01-Right TAL2001 TGAACAGCTC eGFP-TALEN-02-Left...TGGTGCCCAT eGFP-TALEN-02-Right TAL2003 TTACGTCGCC eGFP-TALEN-03-Left TAL2004 TCTGCACCAC eGFP-TALEN-03-Right...TGGGCCAGGG eGFP-TALEN-04-Left TAL2006 TGGAGTACAA eGFP-TALEN-04-Right TAL2007 TGATATAGAC eGFP-TALEN-05-Left...TTCAGCGTGTC eGFP-TALEN-05-Right TAL2009 TAGGTGGCATC eGFP-TALEN-06-Left TAL2010 TGCCACCTACG eGFP-TALEN-06-...TGCAGATGAAC eGFP-TALEN-07-Left TAL2012 TGGCCCACCCT eGFP-TALEN-07-Right TAL2013 TGCACGCCGTA eGFP-TALEN-08-...TTCAAGATCCG eGFP-TALEN-08-Right TAL2015 TGCACGCTGCC eGFP-TALEN-09-Left TAL2016 TAGAGGATCCAC eGFP-TALEN-09...TCGCCCTTGCTC eGFP-TALEN-10-Left TAL2018 TCCGGCGAGGGC eGFP-TALEN-10-Right TAL2019 TCAGCTTGCCGT eGFP-TALEN-11...

  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast...Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1 - Mammalian...then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set ...tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Mammalian Expression PA-GFP 504 517 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian ...with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for C. elegans expression pHT101-mCherry...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...option rtTA On Kowarz 16623 pBI-GFP Expression of your gene of interest & GFP from a bidirectional tet-responsive...Either Lung 11662 pPRIME-TET-GFP-FF3 Lentiviral, miRNA expression (PRIME) system for application in knockdown...cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625...Safe Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for ...generation; contains expression cassette for TetR-P2A-GFP; see article for additional constructs TetR On Geley...inducible promoter at its 3' end, which controls GFP expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA...16542 pBI-MCS-EGFP Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional...

  12. Guide RNA Expression Plasmids for EGFP

    Type
    Collection
    ...Plasmid 47511 pFYF1320 EGFP Site#1 47512 pFYF1320 EGFP Site#2 47513 pFYF1320 EGFP Site#3... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN expression plasmids for EGFP You may... for EGFP CRISPR...technology to modify three different sites in the EGFP reporter gene (Fu et al., Nat Biotechnol. 2013)....

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP S2TAG/E17TAG...E17TAG Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1...68305 Beta lactamase S68TAG Plasmid 69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055...

  14. AAV Molecular Tools

    Type
    Collection
    ...Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals...System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression...expression of diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Jessell , Azim 45580 pAAV-... Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a 5, rg* ...and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression...

  15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...(DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore...the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its fluorescence properties...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... for a researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA ...

  19. Bacterial Expression Systems

    Type
    Collection
    ...many different restriction enzymes. pPro24-gfp contains GFP but all others are empty. pdCas9-bacteria ...fluorophore-tagged MS2 (such as that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest...lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove...Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range...be found here . pAra Series Various Plasmids pBAD GFP Bryan Berger Reporter system for testing hetero- ...AraC-fusion homodimers results in the activation of GFP expression from the araBAD promoter whereas heterodimer...
Showing: 141 - 160 of 267 results