Genome-wide Screening Using CRISPR
Type
Blog Post
...investigating the role of individual genes or entire pathways in a particular disease process. While CRISPR ...
We narrowed to 190 results for: hwa
-
-
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post.... https://www.leicabiosystems.com/us/knowledge-pathway/fixation-and-fixatives-1-the-process-of-fixation-and-the-nature-of-fixatives... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post..." %} References and Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley,... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...like HITI or HDR, which require specific repair pathways to work, PASTE is more specific and efficient ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...intended and may unintentionally remove connecting pathways between other distant regions. They also don’t... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...and the regulation of genes in the biosynthetic pathway. Why plant scientists do transient leaf transformations... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...only Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds especially... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...reading. SHH is involved in the hedgehog signalling pathway, which is a key regulator of all bilateral animal... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes. For example... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...create the substrate for a base excision repair pathway. The team behind AYBEv3 introduced the first deaminase-free... -
Hot Plasmids: Fall 2024
TypeBlog Post...species due to differences in natural DNA repair pathways and spontaneous mutations. Here, the researchers... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...gky332 Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA (2019) A transient... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...pluripotent stem cell generation by the p53-p21 pathway. Nature, 2009. 460(7259): p. 1132-5. PubMed PMID... -
CRISPR Activation: A Practical Guide
TypeBlog Post...simultaneously, CRISPRa can also be used to map molecular pathways and genetic interactions. Additionally, large ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...PMCID: PMC3773023 Howe, S. J., Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, ... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...invasion, a key step in the homologous recombination pathway. The Waldman lab at the Georgetown University School... -
Five Popular Model Organisms, Part 2
TypeBlog Post...2011). As many biological processes and signaling pathways that regulate regeneration and wound healing are... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post... DNA-PKcs-independent, Rad51C-dependent Repair Pathway. Molecular Therapy 21:2205–2216 . https://doi.org... -
Tips for Getting a Faculty Position
TypeBlog Post...postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health, was an Associate...