...key parts of an AAV plasmid. Inverted terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are...Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human...

...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control...in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...

...appears to play a role in many cell processes involving cell membrane dynamics. When they learned there...have some theories on the underlying mechanism, involving the primary cilia. They’ll put a variety of plasmids...gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes with the goal...

...  We started with the idea of a biotechnology investment fund that would apply a percentage of returns... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

...look far for proof that this model of public involvement, research, and consensus can happen. We just ...funded a center for mitochondrial research to investigate the scientific possibility, safety, and efficacy...efficacy of the mtDNA transfer options being investigated; A UK regulatory body tasked two separate non-governmental...

...for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed by Pseudomonas...compounds on these proteins will be tested. Investigating changes in neuronal activity after ischemic ...Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular and cellular etiopathomechanisms...

...study 1: Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are.... Seventeen of these cell lines were used to investigate the susceptibility of cells from different organisms...choose the best experimental model for further investigation of this pathogen. The sharing and distribution...

...relationship with authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always...make the relationship stronger.  If appropriate, invite them to speak for your organization, mentoring ... simple question so I accepted the connection invitation. They now message me frequently and ask me how...

...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...clarification - new and useful information, and invigoration - a renewed energy for continuing the project...

...to make sure the work gets done and done well. Involving your team effectively is now your new measure ...creative thinking. The team members will be more invested in the projects and outcomes. The quality of ...you and you must usually take on all tasks that involve confidential information. No matter who you delegate...

...vector, L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter...Src homology 3 (SH3) domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis...carry out any biochemical and biophysical study involving protein stability or binding experiments. Potential...

...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

... than one?   Multiplex assays Multiplex assays involve multiple antibodies, each with a different signaling...of your fluorophores before buying and try to inversely pair brightness with abundance whenever possible...possible.      Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second...

...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...

...therapies from conceptual design to clinical trials involve identifying a therapeutic gene, finding a means...variety of vector delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...a popular tool in the field as it provides a noninvasive method for manipulating cell activity, especially...provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your...specific circuits. Methods for targeting expression involve tissue specific promoters, AAV serotypes, and the...

...heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics literally... can present without any DNA changes, and both involve the same region of chromosome 15. They both result...positions on histone H3, which are known to be involved in repression and X-inactivation. The K9 reporter...

...discovery: Unless your academic lab is closely involved with clinical trials or has ties to industry, ...work. The number of steps, decisions and people involved is staggering. The process is complicated. If ...important to you, make sure that your project will not involve proprietary compounds or information. Where publication...

... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...educators introduce their students to this otherwise invisible molecular world. For the students, the models ...creating physical models of proteins that are being investigated in their labs. Soon after we began working with...

...which are known to be a part of an organelle or involved in intracellular transport pathways, it is possible...direct molecular interaction without further investigation. One fluorescent microscopy method commonly ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...