We narrowed to 457 results for: nsf

...more stable than the methyltransferase. When a plasmid is lost, the methyltransferase is degraded and/or...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...composed of restriction endonucleases and methyltransferases. Restriction endonucleases cleave DNA at ...specific nucleotide sequences. To counter this, methyltransferases add methyl groups to bases at specific nucleotide...

...size, multimers may exhibit reduced transformation and transfection efficiency compared to the monomeric...plasmid multimerization is to avoid growing the transformed bacterial culture into the late stationary phase...the linearized band, ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric form... Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian...

...specimens. These technologies have the potential to transform neuroscience research by bringing the precision...

...proteins needed for the virus to replicate,  and the transfer plasmid which contains the viral genome. In today...Replication initiates from the AAV genome ITRs on the transfer plasmid, which contain regions of dsDNA. To generate...out this blog post to learn more about the AAV transfer plasmid! References: Naso MF, Tomkowicz B, ...was engineered Learn about the parts of the AAV transfer plasmid  Resources on Addgene.org Learn about...

...1970s by Alex van der Eb in the Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc...replication. To generate a virus, you need only transfect the ‘helper’ plasmids (viral packaging machinery...Protein production  HEKs are extremely easy to transfect in bulk and produce recombinant proteins at high...Resources References Shaw G., et al., Preferential transformation of human neuronal cells by human adenoviruses...

...understand genetic cytoplasmic factors that could be transferred between cells. At the time, these extranuclear...-factors. Like F-factors, R-factors could be transferred between bacteria via cell-to-cell contact; however...pSC102, with EcoRI and selected for E. coli transformants that were resistant to both. When this proved...Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE VIII:...

...December 2015 we held our first intro to bacterial transformation course (read our blog post on it and to see...consisting of over twenty members and volunteers. Transforming citizens into scientists Anyone can become a...dedicated time at BosLab, he became our bacterial transformation guru and instructor for our intro to biohacking...Addgene and are eager to do our first yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami...

...covalently joined, scarless DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications...target region. Assembled plasmids can be directly transformed into E.coli for plating, screening and generation..., generating expression cassettes for yeast transformation can take 5 days. NEBuilder shortens that time...inserted into a linearized cassette, ready for transformation into yeast that same day.  The field of synthetic...

...Wondering how you can develop more transferable skills? See Developing Transferable Skills During Science Training...advantage over other candidates. Write about your transferable skills Some of the skills you developed might...have presented in some public lectures? These transferable skills could be meaningful for your potential...

...due to the ease of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem...construct is also reliably higher than traditional transfection and random genomic integration of a plasmid.... is also generally less toxic to cells than transfections, preserving cell viability. Library delivery... with viral vectors instead of a traditional transfection method. This is because viral systems have a...

...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...

...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the desulfuration of...an enzyme-bound hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides...redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its ...

...the specific reaction you are running. Transformation Transform a few microliters of your ligation reaction...The PCR could fail to produce a product, the transformation may not result in any cells, or all the colonies...fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial strains...

...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...laboratories. The development of the transient transfection platform for assaying transcriptional activity...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

...StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific AAV delivery...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem... viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

...Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded rates of targeted mutagenesis...introduction of DNA into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also...treatment (Stephens et al., 2019). If sufficient gene transfer and knock-in is achieved, producer cells could...

...over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those ...inverted terminal repeats. We use NEB Stable to transform and prep high quality DNA that contains these ... in a protein expression strain, we suggest retransforming the plasmid into a strain meant for DNA purification...to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a Diagnostic Restriction...

...most cases, interested in transferring genes to mammalian cells (i.e. transferring DNA), yet retroviruses...generalize the idea of “transduction” to mean any transfer of material from the virus to the host. In fact...pseudotransduction” — to describe the direct protein transfer of RT, which would actually occur in step 2 during...

...Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring...BiFC constructs are expressed transiently by transfection or stably impacts the success of BiFC experiments...dose-dependent manner. This is straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly...

...brain? Cells of a specific brain area can be transfected by in utero electroporation. To achieve sparse... could reduce the plasmids amounts to reduce transfection efficiency. However, if the cellular structure... it reduces the probability that neurons are transfected with both vectors. Flex vectors are the solution... FLEx vectors guarantees that most cells are transfected with all labeling plasmids and the low concentration...