... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ...PMCID: PMC2770190.  Additional resources on the Addgene blog Find all our optogenetics blog posts Read...

...Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...-12 education.     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic ...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...integration, transposon, and many more! Check out Addgene’s catalog to find trapping tools for your next experiment...Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons in the Lab....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical ...may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs...know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table...cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below. ...inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below...below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell...

...on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled...

... Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...For example, the many resources available on the Addgene website didn't come into being through the actions...develop to reach their full potential in the organization. This will result in more engaged, successful... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others... with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Binning Singletons, Inc. Additional resources on the Addgene blog Browse our blog posts on scientific conferences...

...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...

...CRISPR plasmids for that particular organism available at Addgene. Percentages represent the fraction ... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...available to the academic research community. At Addgene, we’ve helped distribute many of these technologies...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty of producing ...John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you're setting up to...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to ...

...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ...

...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ... up will be easy. Additional resources on the Addgene blog Browse other blog posts on networking Attending...

...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on the...

...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific...

...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...

...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...plasmids or plant expression systems available from Addgene. If you have any questions or thoughts about this...PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed....  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI nucleases for..... Find the plasmids from this publication at Addgene ...