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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 210 results for: pcr

Showing: 141 - 160 of 210 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ..., SacI restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in...can occur in this system if a small contaminating PCR product formed from primer dimers and containing ...clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...and the sequence of gene of interest in separate PCR amplication reactions for recombination in SLIC cloning...the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors with the...or multiply phosphorylated proteins (B40 OTS and pCRT7-GFP). To demonstrate the improvements of the system...

  7. CRISPR References and Information

    Type
    Collection
    ...the tool, you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond... PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers ...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...AAV titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver...

  10. Caltech Systemic Capsids

    Type
    Collection
    ...including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and...

  11. Cloning

    Type
    Guide
    ...add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...a single adenosine (A) overhang on the 3' end of PCR products. The complementary thymine (T) comes from...an incredibly quick and easy way to clone a fresh PCR product into a plasmid. The major disadvantage of...are also available, allowing for rapid cloning of PCR products into donor plasmids without the need for...used, so if properly designed, any insert fragment (PCR product or synthesized oligo) with appropriate overhangs...add). Complementary overhangs are built into the PCR primers for the insert, based on the destination ...

  14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...and these rare events are easily detectable using PCR. The system also functions in S. pneumoniae and can...created two “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes ...array, while pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays...pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting the gene of interest...machinery are first electroporated with pCas9. Then, pCRISPR is introduced along with an oligonucleotide repair...advantages including shorter plasmid curing time. pCRISPomyces for editing in Streptomyces  Streptomyces bacteria...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...annealed synthetic oligos - eliminating the need for PCR or additional molecular cloning steps during plasmid...occurred at higher frequency and could be detected by PCR while large deletions were relatively rare. The vectors...target G-protein coupled receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors...Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307...are unknown. Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts...allows researchers to interrogate the druggable human GPCR-ome using a quick, affordable, and ubiquitous reporter...recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression. In a twist...

  17. Video Library

    Type
    Protocol
    ...commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA...maintaining sterility while working. AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-...adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel...
Showing: 141 - 160 of 210 results