...steady stream of papers describing new photosynthetic or photosensory molecules that come from basically ...would be helpful to have molecules with better two-photon properties, so you could activate single cells ...

...sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

... up the phone and having a discussion. By only relying on email and never picking up the phone you don...advice someone has given you? Katz: Pick up the phone. I think that there is a tendency to go back and...

.... 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex... complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek...

...grow over time, but being interested in the same phosphate of your favorite kinase is a good start. Check...next to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...meals, sit with people you don’t know and put your phone away (did I say that already). Start with, “Is this...

...Because roGFP2 contains two cysteines near the chromophore that are susceptible to redox modification, the...excitable. The mutants are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing...sensor that shows a favorable outcome are then photoconverted, sorted, and sequenced to identify the mutants...

.... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

... microbes? Try your hand at photography and participate in a science photo competition. Or nominate yourself...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

...the opsins will receive sufficient quantities of photons to become activated. Light is able to penetrate...between patch cable and implant. A steady stream of photons from the laser to the patch cable to the ferrule...insensitive. If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity...

... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

...further simplifies lab work if you like to bring your phone in the lab, though, honestly, this still grosses... out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the lab...but is there a sterile, clean way to bring your phone into the lab? Maybe having a dedicated lab-use tablet...

...as neural development. Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration...Roger Karess. "Myosin light chain–activating phosphorylation sites are required for oogenesis in Drosophila...

... FP scaffolds with either intact or darkened chromophores. These probes are both intrinsically fluorescent...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities...

...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

...that prefer a more economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach...812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination of cell cultures...Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C., Drexler, H.G. “Detection of mycoplasma contaminations...

...multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq ...not always achievable. Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate...

...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...

...after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation. Generally, I...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear...