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We narrowed to 407 results for: pho;

Showing: 141 - 160 of 407 results

  1. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

  2. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...often used to monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow... Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources...

  3. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited to autophagasomal membranes...Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology...protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources...

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...

  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  9. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...grow over time, but being interested in the same phosphate of your favorite kinase is a good start. Check...next to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...meals, sit with people you don’t know and put your phone away (did I say that already). Start with, “Is this...

  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  11. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...the opsins will receive sufficient quantities of photons to become activated. Light is able to penetrate...between patch cable and implant. A steady stream of photons from the laser to the patch cable to the ferrule...insensitive. If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity...

  15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...further simplifies lab work if you like to bring your phone in the lab, though, honestly, this still grosses... out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the lab...but is there a sterile, clean way to bring your phone into the lab? Maybe having a dedicated lab-use tablet...

  16. MXS Chaining

    Type
    Blog Post
    ... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

  18. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... FP scaffolds with either intact or darkened chromophores. These probes are both intrinsically fluorescent...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities...

  19. Viral Production at Addgene

    Type
    Blog Post
    ...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...that prefer a more economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach...812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination of cell cultures...Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C., Drexler, H.G. “Detection of mycoplasma contaminations...
Showing: 141 - 160 of 407 results