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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 400 results for: pho;

Showing: 141 - 160 of 400 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  6. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...grow over time, but being interested in the same phosphate of your favorite kinase is a good start. Check...next to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...meals, sit with people you don’t know and put your phone away (did I say that already). Start with, “Is this...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  9. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  11. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...the opsins will receive sufficient quantities of photons to become activated. Light is able to penetrate...between patch cable and implant. A steady stream of photons from the laser to the patch cable to the ferrule...insensitive. If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity...

  12. MXS Chaining

    Type
    Blog Post
    ... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

  13. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...further simplifies lab work if you like to bring your phone in the lab, though, honestly, this still grosses... out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the lab...but is there a sterile, clean way to bring your phone into the lab? Maybe having a dedicated lab-use tablet...

  14. Viral Production at Addgene

    Type
    Blog Post
    ...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... FP scaffolds with either intact or darkened chromophores. These probes are both intrinsically fluorescent...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...that prefer a more economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach...812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination of cell cultures...Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C., Drexler, H.G. “Detection of mycoplasma contaminations...

  18. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq ...not always achievable. Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation. Generally, I...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear...
Showing: 141 - 160 of 400 results