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We narrowed to 416 results for: plasmids 101

Showing: 141 - 160 of 416 results

  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101: Viral...Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook, out...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors... Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W. (2014)...

  2. Inntags: Innovative Protein Epitope Tagging

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    Blog Post
    ...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with...in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization of...

  3. What's New in CRISPR - March 2020

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    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment?...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019). 10.1016/j.molcel.2019.03.014 Zetsche, Bernd, HGootenberg...CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016/j.cell.2015.09.038 Harrington, B., Lucas, Burstein...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...exact Cas9 plasmid used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and...Guide Addgene’s CRISPR 101 eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose...find a link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References...lists on every page you can find direct links to plasmids from the article if they're available through ...choose for my CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) The PAM Requirement and Expanding CRISPR...

  8. Lucky Thirteen: Guest Contributors of 2023

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    Blog Post
    ... Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila ...Marjorie Buss and Mengtong "Tom" DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart  Created ...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj; ...

  9. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Molecular Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID: 4355596 King...virus. Virology, 46(3), 866–876. https://doi.org/10.1016/0042-6822(71)90087-0. PubMed PMID: 4332980...

  11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC.... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...

  12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post
    ...blog Plasmids 101: Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden...through your plasmid stocks. To avoid cross-contamination, we recommend retrieving plasmids from your original...Calculation for the plasmid volume for MoClo experiments. The term ‘insert’ refers to plasmids carrying each...will find some useful tips to help you make your plasmid construction with MoClo a walk in the park.  Before...promoter, a coding sequence, a terminator, or other plasmid part? Do you want to fuse your protein to any tags...restriction enzymes, and it will create the resulting plasmid map and identify any mismatches that are preventing...Level 0, Level 1, and Level 2 assemblies using the plasmid sequence files provided with every Addgene MoClo...

  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...blog:  Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies 101: Introduction...important collection.  Creating the Plasmids To begin constructing the plasmids for expression of mAbs, the Trimmer...expression plasmids were DNA sequenced to determine the VH and VL sequence. These plasmids have been deposited...scientific community with the plasmid sequences available from each plasmid webpage. Importantly, the methods...NABOR are recombinant antibodies expressed from plasmids that have been deposited with Addgene. Here, we... first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based off... to create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light...

  16. Adenovirus Plasmids

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    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class ...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...special collections page Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ... gene expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging...this case, plasmid copy number is the input and gene expression is the output. The input, plasmid copy number...stationary phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, ...RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid by altering...genome or a plasmid. Thus, a stabilized promoter can allow a system to be moved from a plasmid to the genome...PMC3163176. Resources on the Addgene Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology...

  18. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...CRISPR blog posts Find CRISPR Plasmids at Addgene  ... crispr 101...Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our...

  19. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...affect nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop...addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on the Addgene...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors CRISPR 101: Cytosine Transversion Editors...CRISPR plasmids for base editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook...Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N., Wei, Y....
Showing: 141 - 160 of 416 results