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TypeProtocol... to the appropriate well. Pro-Tip When possible, skip one lane before loading samples. Load 20 µL of each...
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A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...programming, you’d be wise to start working on these skills. Neuroscientists use lots of cool tools While walking...there’s likely some way you can use the tools and skills you’ve developed in your current work to make new... -
A Deep Dive into BioBuilder
TypeBlog Post...question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...Keratinocytes are the main source of IL-36 cytokines in the skin making it important to study what is happening within... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea. Pro tip: ...accomplishments in both the technical and “plus skills” areas. Include a professional picture. It doesn... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...developing reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways...Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about the 2018 Minisymposium... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...your professional presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes...especially excellent in coaching for communication skills. Industry transition is also an area of specialty... -
Engaging with science and society at pgEd
TypeBlog Post...also beneficial to hone your non-technical writing skills by writing for science-outreach organizations or...work to a non-technical audience is an important skill for every scientist. After all, scientific research... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...and use them as-is, saving time and effort but risking the potential of them not working, or spend a few... expected module order. (It’s also why you can’t skip a module entirely, but instead will have to replace... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...through on your commitments. Assertiveness is a skill that will help you to resolve both personal and ...excellent resource to learn more about assertiveness skills is Dale Carnegie’s book “How To Win Friends and... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...Volunteer at a professional organization to expand your skill set Resources on the Addgene website Become of... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post... take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293 cell culture.... 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production of recombinant adeno-... -
Kit Free RNA Extraction
TypeProtocol...was adapted from (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...Results from a survey conducted by Addgene in 2021 asking scientists what data would improve our AAV Data... your data free. Nature, 5–7. O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure. If you expect a lot of...children but are interested in testing your SciComm skills or are interested in a career in education. Maybe... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...collecting fibroblasts, which require a more invasive skin biopsy procedure. This becomes a major benefit when...patients, specifically children and patients with skin disorders for research and clinical trials, as it... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...network at the meeting. We found our mentors by asking for help with the program on social media. Many...Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post... References and Resources References Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, ...Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction:...Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV Transductifon Is Mediated...